FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n01_ncm10-18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n01_ncm10-18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5664050
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATG3589966.338150263504031TruSeq Adapter, Index 21 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTGTG451320.796814999867586TruSeq Adapter, Index 21 (97% over 46bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTT280460.49515805827985276TruSeq Adapter, Index 21 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCGCGTATG257710.4549924523971363TruSeq Adapter, Index 21 (97% over 42bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCG226260.39946681261641404TruSeq Adapter, Index 21 (100% over 39bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTC100900.17814108279411375TruSeq Adapter, Index 21 (100% over 38bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGC91590.1617040810021098TruSeq Adapter, Index 21 (97% over 48bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTAT74760.13199036025458816TruSeq Adapter, Index 21 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGC66200.11687749931586056TruSeq Adapter, Index 21 (98% over 50bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTAT63410.11195169534167247TruSeq Adapter, Index 21 (97% over 48bp)
AACTCCACCCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTT61860.10921513757823466TruSeq Adapter, Index 21 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGGATG57070.10075829132864293TruSeq Adapter, Index 21 (97% over 45bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGA36550.058.556691
GCGTGGA158750.055.2922860
GGGTGGA132300.053.1195760
GTGGAAA300150.053.01970762
CGTGGAA169800.051.98261361
ATCGCGG19400.051.05465741
GGTGGAA140100.050.5368861
TGGAAAA381650.049.91415863
GTCGGAA40250.049.408522
CGTGGTG148650.047.300552
CTCGTGT78050.047.17343
GCCGTGG169600.047.1325550
CCGTGGT165050.046.9687451
CGCGGGT11600.046.7653743
TCTCGTG79600.046.21046442
TGCCGTG210000.045.96483249
TGGTGGG144750.045.72183654
TGGGGGT178000.045.3605557
TCGCGTG29400.044.8791642
GATCTCG711750.044.25042340
Produced by FastQC (version 0.11.5)