FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n01_ncm10-17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n01_ncm10-17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7542580
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGGGGGTG972571.289439422584845TruSeq Adapter, Index 20 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGGGGGGG286820.3802677598381456TruSeq Adapter, Index 20 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCGGGGGTG180430.23921522874135906TruSeq Adapter, Index 20 (97% over 42bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGG111650.1480262721774247TruSeq Adapter, Index 20 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATGGGGGGTT101290.13429091902240348TruSeq Adapter, Index 20 (97% over 41bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCC80130.10623685794515934TruSeq Adapter, Index 20 (100% over 39bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTGGC144350.050.066946
GGTGGCG136300.049.6087947
ATCGGGG50600.049.52333541
GTGGCGG137200.049.30888448
TCGGGGG45150.047.5171242
CGGGGGT38700.046.75488343
CATCGGG56250.045.85559540
TGGCGGG162350.044.99016649
AGTCGGA20500.042.7194141
GCGGGGT82100.041.69107451
GGCCATC76650.041.23091537
GCCATCG74550.040.93702338
CGGGGTG91000.040.9211352
GGCGGGG211450.038.0024550
GGGTGGA51700.037.3676660
CCATCGG68400.037.09618839
ATCGCGG14700.036.18875541
AAAAAAG343450.034.9932470
GGTGGGA45700.034.76858561
ATGGGGG324000.033.16200341
Produced by FastQC (version 0.11.5)