FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n01_ncm10-16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n01_ncm10-16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7301019
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGGGGGTG4135715.664565453123735TruSeq Adapter, Index 19 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGGGGGGG852701.1679191630647723TruSeq Adapter, Index 19 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGGGGGTT442260.605751060228716TruSeq Adapter, Index 19 (97% over 42bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGA300090.4110248172207195TruSeq Adapter, Index 19 (100% over 37bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAA291220.3988758281549466TruSeq Adapter, Index 19 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGGGTGTG228030.31232626568976196TruSeq Adapter, Index 19 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGCGGGTG225850.30934038111666334TruSeq Adapter, Index 19 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGGGGTTG204730.28041291222499215TruSeq Adapter, Index 19 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGGGGATG188620.2583474991641578TruSeq Adapter, Index 19 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTGGGGTG141030.19316481713032113TruSeq Adapter, Index 19 (97% over 43bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAA132130.18097473790987256TruSeq Adapter, Index 19 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCGCGGATG87900.12039415319971089TruSeq Adapter, Index 19 (97% over 42bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGA40000.059.985421
GGTGGCG441900.056.8497647
GTGGCGG472600.055.91906748
TGGCGGG509050.055.24953549
GGGTGGC489100.054.6837846
CGGGGGT630050.054.29333543
GGGTGGA185050.052.82396760
GGTGGGA136100.052.7677361
GGTGGAA192400.051.4608661
GCGGGGT335000.051.10822751
GTGGGAA149200.050.363162
TCGGGGG825300.049.9978342
GTGGAAA231300.049.85732362
TGGGAAA153250.049.8542863
ATCGGGG932150.048.88110441
CGGGGTG407050.048.21802552
ATGCCGG27950.048.20899648
GCGTGGA32250.047.96690860
AATCGGG1014000.047.7760340
TGGGGGT278300.047.57428757
Produced by FastQC (version 0.11.5)