FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n01_ncm10-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n01_ncm10-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5894757
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGGGGGTG5520939.365831365058815TruSeq Adapter, Index 18 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGGGGGGG976891.657218440047656TruSeq Adapter, Index 18 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGCGGGTG583840.9904394701935975TruSeq Adapter, Index 18 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGGGGGTT490080.8313828712532171TruSeq Adapter, Index 18 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGGGTGTG468690.7950963881971725TruSeq Adapter, Index 18 (97% over 42bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCC446190.7569268758661298TruSeq Adapter, Index 18 (100% over 37bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGC423420.7182993293871146TruSeq Adapter, Index 18 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGGGGATG287930.4884510082434272TruSeq Adapter, Index 18 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGGGGTTG261750.4440386601177962TruSeq Adapter, Index 18 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTGGGGTG246010.41733696571376905TruSeq Adapter, Index 18 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGCGGATG206920.35102379962397096TruSeq Adapter, Index 18 (97% over 45bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCG197200.3345345702969605TruSeq Adapter, Index 18 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGCGTATG152120.2580598318132537TruSeq Adapter, Index 18 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGCGTGTG130090.22068763818423728TruSeq Adapter, Index 18 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGGGTATG118110.2003644933964199TruSeq Adapter, Index 18 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTGGTGTG99220.16831906726604676TruSeq Adapter, Index 18 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATG96070.16297533553970078TruSeq Adapter, Index 18 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG82160.13937809480526508No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGGGGATT75300.12774063460122276TruSeq Adapter, Index 18 (97% over 42bp)
AACTCCACCCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCC72490.1229736866167681TruSeq Adapter, Index 18 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGGGTG70210.11910584270055577TruSeq Adapter, Index 18 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGGGTTTG68240.1157638898431267TruSeq Adapter, Index 18 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGCGGTTG66240.11237104430258957TruSeq Adapter, Index 18 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGGGTGTT63710.10807909469381012TruSeq Adapter, Index 18 (97% over 42bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCGGGGTG61500.10433000037151657TruSeq Adapter, Index 18 (97% over 42bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGA49750.060.9735031
GGTGGGA180150.058.20483861
GCGTGGA53600.057.52579560
GTGGGAA190300.056.9946862
TGGGAAA194450.056.71422263
GGTGGCG639350.056.6131647
ATGCCGG51150.054.9441548
GTGGCGG711500.054.90093248
GGGTGGA301250.054.48755360
GGTGGAA304000.054.43213761
GTGGAAA363400.054.3856662
TGGCGGG770150.054.38279349
GGGTGGC705050.054.3458546
GGATGGC50150.054.01589246
AAAAGGG129500.052.700770
CGCGGAT35700.052.64505843
GATGGCG45300.052.3821147
GTCGGAA55700.052.2343032
CGGGGGT862700.052.18359443
CGGGGAT54300.051.4345143
Produced by FastQC (version 0.11.5)