FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n01_ncm10-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n01_ncm10-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6241425
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATG2613414.187200839551865TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTGTG850661.362925934381972TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCGGGGGTG408360.6542736634662758TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCGCGGGTG339360.5437219865655679TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCGCGTATG330570.5296386642473474TruSeq Adapter, Index 3 (97% over 37bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAG316800.5075763948136844TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCGCGTGTG296940.4757567382448719TruSeq Adapter, Index 3 (97% over 37bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAG287480.46059994312196334TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGGATG252140.4039782581702095TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGGGTG249400.3995882350585003TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGTGTG202050.3237241495331595TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCGCGGATG166670.2670383766527676TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGTATG159590.25569481328382543TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGGTG159380.2553583516584754TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCGGGTGTG144670.2317900159018173TruSeq Adapter, Index 3 (97% over 37bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGA136640.21892436422772046TruSeq Adapter, Index 3 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGGATG67180.10763567614767461TruSeq Adapter, Index 3 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG65500.10494398314487477No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCGGGTATG64390.10316554312516774TruSeq Adapter, Index 3 (97% over 37bp)
AGTCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC64270.10297327933925346TruSeq Adapter, Index 3 (97% over 35bp)
AACTCCACCCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAG63610.10191582851672494TruSeq Adapter, Index 3 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGA40850.060.1175271
GCGTGGA220850.059.20474260
GTGGAAA511800.057.12697662
ATCGGGG83150.056.82226641
CGTGGAA232550.056.78288761
CGGGGGT61600.056.41772543
GGGTGGA282850.056.03935660
GGTGGAA291600.054.88588761
GATCGGG122250.054.53724340
TGGAAAA579550.054.1204163
GGTGGGA87700.053.3953661
TCGCGGG54050.052.6431542
CGTGGTG237150.052.30191852
CGCGGGT50100.052.183143
GCCGTGG221650.052.0592950
ATCGCGG87000.051.93426541
CCGTGGT225550.051.3918851
TGCCGTG252900.051.20353749
GTCGGAA46100.050.7377662
TCGGGGG77900.049.73443242
Produced by FastQC (version 0.11.5)