FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n01_ncm10-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n01_ncm10-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5944307
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATG1327592.233380611061979TruSeq Adapter, Index 13 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCGGGGGTG1186361.99579194008654TruSeq Adapter, Index 13 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTGTG897311.5095283604968586TruSeq Adapter, Index 13 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCGCGGGTG765471.287736316445298TruSeq Adapter, Index 13 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGGGTG560790.9434068597062701TruSeq Adapter, Index 13 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTGGGGTG299670.5041294132352181TruSeq Adapter, Index 13 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCGCGTGTG296190.4982750722666242TruSeq Adapter, Index 13 (97% over 42bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTC277450.46674910969436806TruSeq Adapter, Index 13 (100% over 37bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAA258570.4349876276578582TruSeq Adapter, Index 13 (100% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGGATG255860.43042864374266004TruSeq Adapter, Index 13 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCGCGTATG189670.31907840560724743TruSeq Adapter, Index 13 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCGCGGATG168100.28279158529328985TruSeq Adapter, Index 13 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTGGTGTG148150.24923006163712608TruSeq Adapter, Index 13 (97% over 43bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCGGGTGTG123130.20713936881120035TruSeq Adapter, Index 13 (97% over 42bp)
ACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCA119210.20054482381209449TruSeq Adapter, Index 13 (100% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTTTG96180.16180187194234755TruSeq Adapter, Index 13 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCGGGGTTG83540.14053782888400615TruSeq Adapter, Index 13 (97% over 42bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72030.12117476435857032No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCGCGGTTG66680.11217455626030082TruSeq Adapter, Index 13 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTGGTATG65750.1106100341049007TruSeq Adapter, Index 13 (97% over 43bp)
AACTCCACCCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTC63480.10679125422021439TruSeq Adapter, Index 13 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGGTTG62330.10485663004955834TruSeq Adapter, Index 13 (97% over 45bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCGCGGGTT60510.1017948770142592TruSeq Adapter, Index 13 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATT60120.10113878707812365TruSeq Adapter, Index 13 (97% over 48bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGGA36350.060.5115431
GCGTGGA155350.059.04863760
ATCGGGG225000.057.25816741
CGGGGGT174050.056.645743
GTGGAAA477400.055.50408662
AATCGGG260000.055.37899440
TCGGGGG206750.055.25336542
GGGTGGA309150.055.144660
GGTGGCG253800.054.92534647
CGTGGAA168900.054.72589561
GGTGGAA319950.053.78636661
GTGGCGG308600.052.56636448
GGTGGGA107200.052.56361461
CGCGGGT118500.052.54261443
GTCGGAA40150.052.492862
TGGAAAA532500.052.1269763
AAATCGG280550.051.84649339
TCGCGGG126800.051.64266642
ATCGCGG169650.051.6163841
CTCGGGT82550.051.5125843
Produced by FastQC (version 0.11.5)