FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n01_ncm10-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n01_ncm10-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6758862
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGGGGGTG502340.7432316268626287TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGGGGGTG219210.3243297466348625TruSeq Adapter, Index 12 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGGCGGGTG138990.20564112716016394TruSeq Adapter, Index 12 (97% over 41bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCGCGGGTG132200.19559505727443466TruSeq Adapter, Index 12 (97% over 45bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTG72910.10787318930316969TruSeq Adapter, Index 12 (100% over 37bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTA68230.10094894673097336TruSeq Adapter, Index 12 (100% over 39bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGGG58850.052.2749540
ATCGGGG50000.052.07798441
GGTGGCG122050.051.61619647
GGGTGGC131100.050.45571546
TAATCGG62300.050.4474939
CGGGGGT38950.050.4088343
TCGGGGG44750.050.36676442
GTAATCG110450.049.3371638
GTGGCGG145300.049.25824448
GGGTGGA82200.047.81445360
CGCGGGT20850.047.1684443
ATCGCGG32300.046.59262541
AATCGCG48200.046.47123340
GCGGGGT118850.046.439151
TGTAATC161800.045.38143537
TCGCGGG23650.045.28366542
CGGGGTG153800.044.0782752
AGTCGGA19700.042.8482551
TAATCGC53900.042.33602539
GGTGGGA47250.041.47987761
Produced by FastQC (version 0.11.5)