FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n01_ncm10-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n01_ncm10-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5516204
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG504940.9153758635467434TruSeq Adapter, Index 11 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTGTG337910.6125770548007289TruSeq Adapter, Index 11 (97% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCGCGTGTG143810.2607046439906864TruSeq Adapter, Index 11 (97% over 46bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCGCGTATG73250.13279059295123966TruSeq Adapter, Index 11 (97% over 49bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCT63960.1159493013673896TruSeq Adapter, Index 11 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCGCGGGTG63560.11522416502362856TruSeq Adapter, Index 11 (97% over 45bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGGA51200.050.6523160
GGGTGGA65650.048.246460
AGTCGGA15900.045.8246571
GTGGAAA134750.043.29762
TGGGGGT85900.041.72129457
GGTGGAA77100.041.71691561
GGGCGTG47800.041.4418958
GCCGTGG48000.041.41504350
GTGGGCG47200.040.33740256
CGTGGTG53600.039.89582452
TGCCGTG56300.039.7231349
TGGGCGT49550.039.69571757
CGTGGAA66000.039.66510861
GTGGGGG114800.039.4191956
TCGCGTG28600.039.4040542
CCGTGGT49300.039.32908651
GGGGGTG94900.039.23976558
CGCGTGT28000.038.87347443
TGCCGGG54750.038.48250649
GGTGGGG104100.037.99082655
Produced by FastQC (version 0.11.5)