FastQCFastQC Report
Tue 15 Sep 2020
HH7CGAFX2_n01_ncm10-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH7CGAFX2_n01_ncm10-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5745575
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGTG1154742.009790142849062TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGGG289370.5036397575525513TruSeq Adapter, Index 10 (97% over 42bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGGTT79120.1377059737276078TruSeq Adapter, Index 10 (97% over 42bp)
AACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGC72510.12620146808630991TruSeq Adapter, Index 10 (100% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGGGGATG62660.10905784016395227TruSeq Adapter, Index 10 (97% over 42bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGGG131350.053.47697449
GGGTGGC131500.053.282946
GGTGGCG126550.052.93334247
GTGGCGG131650.051.83979448
AGTCGGA20850.049.0582431
CGGGGGT187250.048.4091543
GCGGGGT73050.044.4128951
GGGTGGA42150.043.01121560
TGGGGGT53950.041.25866757
GGCGGGG185050.041.19251650
GGTGGGA43100.040.35790661
GTCGGAA25250.039.5201152
TCGGGGG287550.039.48362442
CGGGGTG89200.038.8828752
ATCGGGG325500.038.105941
TATCGGG346350.036.80224240
TTATCGG359800.035.49459539
AAAAAAG320100.035.05317770
GGGAAAA233600.034.84868664
GCGGGGG144850.034.40654451
Produced by FastQC (version 0.11.5)