FastQCFastQC Report
Sun 15 Jan 2017
HH75LAFXX_n01_undetermined_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH75LAFXX_n01_undetermined_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13153094
Sequences flagged as poor quality0
Sequence length145
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3773952.869248862663036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAACCG12100.048.2406967
CTGAACC12800.047.7744456
TTTCCCT9400.022.1773571
TTCCCTA4751.8189894E-1221.943742
TCCGGGA1304.630723E-621.3840956-57
AAGGGGG27300.021.13040222-23
AACCGCT12750.020.1657549
AAAAGGG29400.020.09420-21
AAAGGGG29800.019.00787722-23
GAGGACC1501.5923639E-518.535486138-139
TTCCCTG4958.385541E-1018.249512
TTACAGG3250.018.177124-25
CCCGTGA1752.9978037E-617.87234998-99
AGGGGGG31650.017.78690324-25
GGGCACT2801.9748967E-417.3725266
TCCCTAC5803.8198777E-1016.7730273
CCTACAC5955.475158E-1016.3504895
TCACGGA9350.016.3504878
TCCCGTG1503.3386587E-416.21750398-99
ACCCGTT4301.1867942E-616.1603668
Produced by FastQC (version 0.11.2)