FastQCFastQC Report
Sun 15 Jan 2017
HH75LAFXX_n01_tpq1ss_9_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH75LAFXX_n01_tpq1ss_9_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3799592
Sequences flagged as poor quality0
Sequence length145
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAACTACAAATGC255380.672124796557104No Hit
TATTTGATGGAAGCAAGTACTTTTTAGATACCCAGTAAAAGTCTCCATAT249880.657649558163087No Hit
TATGAACAACAATTGTTGTAGTCGCAACTACGGTAATTGGTCCTAAAAAA231760.6099602273086163No Hit
TATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAACTACAAAT205090.5397684804052646No Hit
TTGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAA202580.5331625079745405No Hit
TCTCTGGAACAGCTGATGAAGCAGGTGTTGTTGTCTGTTGAGAGTTAGCC159820.42062410911487336No Hit
TGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAG128080.33708882427376413No Hit
TTGAAATTGTAGGTCACCAATAGCGTTAATTGGTATATTTCTTTTTTGAG112240.29540013769899504No Hit
TCATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAACTACAAA106540.28039852699974105No Hit
TAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAGGAG101550.26726553798407826No Hit
TAGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAAGA99560.2620281335469703No Hit
TCCTAAGCGCAGCATATCATGAAAGAGCGGTGAGACATGCTTGGAAGGGG97250.25594853342148316No Hit
TCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCACATTCT89380.23523578321040786No Hit
TATGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTG88590.23315661260472176No Hit
TGTGCTAGATCTCTTCTTTGATGGAAGAATATTAGATTCAGATATGTTGG86680.2281297570897086No Hit
TCTAAGCGCAGCATATCATGAAAGAGCGGTGAGACATGCTTGGAAGGGGA84950.22357663664940866No Hit
TAAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCCCT74870.19704747246546472No Hit
TCTTATAAGGGTTCGTGATGCTCCTGAATCGAGAAGGAGGTGTCCAGGGA73890.1944682481698035No Hit
TGTTATTAGATGTGGATACATTGTGAGCCCTGGCTGTTTTCGATTTCGAA72890.19183638664361857No Hit
TGGGCCCCAAAAATCAGTTTAACCCAAAAATCCCCAAAGGAATCAAGGGA69770.18362497868192165No Hit
TCAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTTGGCGT67970.1788876279347888No Hit
TTGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTGG67360.17728219240381599No Hit
TCTTTAAATGGGTCAGGGAATTCGGTAGGAGATTCTGGAGGTAGATCAGG66960.17622944779334201No Hit
TATTTGCGTGTACTTTCACTTGTATGGACATTATTGATGGTGGGTACGGA65920.17349231180610972No Hit
TGTTGACATTGAGAATATAGATAGTTATAAGAAGACAAGGATGATTGCCA65310.1718868762751369No Hit
TCATCGATTGAAGGTGAACGGTGTATAATCCTTTTCTCAGTCTCTTGGTC63930.16825490736900173No Hit
TGTAGAGCGTAGCCTGGGATGCCACGAGGATGTATTTTGGAGTTAGGGTT63240.16643892291593412No Hit
TCTTATGTATAATTCTTCTTTGATGTCTGCATACAAATATGCCGAAGATA60670.1596750387936389No Hit
TTGCTACTTTCACGCACAGTAGGGTTGAGTCTGATGCACCACTGACATTG58660.1543849971260072No Hit
TGTCAAGATCATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAA58130.15299011051712919No Hit
TGCCTTCCATTGCCTCCTTTTTTTCTCTTCCAGAACTCTCTCAGCAGTAT56640.1490686368431137No Hit
TTAGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAAG56180.1478579805410686No Hit
TCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCTC54800.14422601163493343No Hit
TATTAGATGTGGATACATTGTGAGCCCTGGCTGTTTTCGATTTCGAATTA54010.14214684102924735No Hit
TGTCAGTCAGGACCATAGCTACTGGCAATTATCGAGATGCTGCTGAAAGA49800.13106670400400885No Hit
TTGCTAGATCTCTTCTTTGATGGAAGAATATTAGATTCAGATATGTTGGG49580.13048769446824818No Hit
TGGCCCCAAAAATCAGTTTAACCCAAAAATCCCCAAAGGAATCAAGGGAT49040.12906648924410832No Hit
TGCATGATGGGGGTTCTCTGGAACAGCTGATGAAGCAGGTGTTGTTGTCT47860.12596089264321012No Hit
TCCACCGTTCAAAACGTTCAAGAATGGAACTGGCAAAACGTATGGAGAGG47570.1251976528006165No Hit
TGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAGGAGTTT47120.12401331511383328No Hit
TCTCGTTGGCACTAGTGAGTTTATACCTGCATTAGCCAGGTAGCAATAGT47070.12388172203752404No Hit
TCAAATATTTGAAAAGTGTTATACAAGAAGGTGAGTTCATCATCAGTGAT46800.1231711194254541No Hit
TAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTTGGCGTT46390.12209205619971829No Hit
TGTGATAACAACCTTCTTGGCACCAGCGTCAATGTGCTTTTGAGCAGTGT46370.12203941896919458No Hit
TGGCATGATGGGGGTTCTCTGGAACAGCTGATGAAGCAGGTGTTGTTGTC46210.12161832112500501No Hit
TCTCCCGTAACACTATCTCTGATACCCTACCCTTTTCTTGCAAACCGCCA46030.12114458605029171No Hit
TCTTGCTGTCTCTAGCAGCTTCTAAAGCAGCGACGGCAGTAGAAGAAGAG45980.12101299297398248No Hit
TGTAGCAATGTCAGTGATCATAGCAGTGGTTTCAGAAATGTTGGCAGACA44280.11653882837946812No Hit
TCTGTGATTGTGGCCCAGGTGGAAAGTACATAGGCGACATTTGATAAGGT44260.11648619114894442No Hit
TGTAAAGGAACATTTAATTTATGCATACCACCCGAACCGGTACTCTCGAT42880.11285422224280922No Hit
TCGGGTCAAGATCATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAA42740.11248576162914334No Hit
TATGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCA42500.11185411486285896No Hit
TACAGAAAGCTAAAAAAAAGAAAATAGAAAACAACCAGAAGAAAACAAAA42120.11085400748290868No Hit
TGCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCT42070.11072241440659944No Hit
TCTCTAAAGAAGAGCGGTCACCGAGAGTACTAACGATGGGTTCGTAAGCG42030.11061713994555206No Hit
TGTGGAATCGGTTGGACTCACAGCTTTTGAAAGGACATTTCTCGGTTGCT41660.10964335118086363No Hit
TCTGACATATTTTTTAGTGGATGTCATATCAGAGTCCGCTGAGGATGAAT40570.10677462211732207No Hit
TGATGGAAGCAAGTACTTTTTAGATACCCAGTAAAAGTCTCCATATTTTA39930.10509023074056373No Hit
TGGGTCAAGATCATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAA39270.10335320213328168No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTAAG15850.0101.71231
TCGGGTC10700.096.764761
TATCGCG31400.096.0450061
TCGGGCC4950.088.440271
TGGGCCC16200.087.50441
TCTAAGC17050.076.213511
TGGCCCC14200.075.361081
TCGGGAC4550.074.8340761
TATGAAC47600.071.386591
TCGGGCT7800.067.707021
TCGCCGG5600.067.007051
TCGCGGC3250.062.0053751
TCCCGCA13850.055.6913721
TCCCGTT12500.055.0351221
TCCAAGC30700.053.644448
TCGCGCG1950.053.452911
TTGCTAG18300.052.78111
TCGGCTC4850.051.5793081
CTCCCGT17000.051.5020982
TGTCCGC4750.051.2022631
Produced by FastQC (version 0.11.2)