FastQCFastQC Report
Sun 15 Jan 2017
HH75LAFXX_n01_tpq1ss_8_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH75LAFXX_n01_tpq1ss_8_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2980666
Sequences flagged as poor quality0
Sequence length145
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCCCT124950.41920161467269396No Hit
TTGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAA115560.3876985881678793No Hit
TCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCACATTCT78820.2644375451660803No Hit
TATGAACAACAATTGTTGTAGTCGCAACTACGGTAATTGGTCCTAAAAAA63920.21444871716589514No Hit
TCAGAAATGATCAACACCAGGCGCCCAGCAACGTTCAATCTATCTACGCT58900.19760684357120187No Hit
TCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCTC58670.19683520394435336No Hit
TACTCGATCCAGCACGGGAAAGAGCAATCGTCATAACTATGGTTTAGATT58060.19478868145575517No Hit
TGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAG55810.1872400329322373No Hit
TGGGCCCCAAAAATCAGTTTAACCCAAAAATCCCCAAAGGAATCAAGGGA51380.1723775827281554No Hit
TAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAGGAG51130.17153884400332006No Hit
TAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCCCTTG47370.1589242135817968No Hit
TCTCCTTTTAAAAATTAAAAACAGCAAATAGTTAGATGAATATATTAAAG46340.1554686100354753No Hit
TGGCCCCAAAAATCAGTTTAACCCAAAAATCCCCAAAGGAATCAAGGGAT45950.1541601776247322No Hit
TCTCCCGCCTCCGGGCAGACAGCTCTGCCCAGGACAAATTTACGACGGAG43230.1450347002985239No Hit
TCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAACTACAAATGC42630.1430217273589191No Hit
TCTCTAAAGAAGAGCGGTCACCGAGAGTACTAACGATGGGTTCGTAAGCG42410.14228363728106402No Hit
TCCCGTTCCTACCAAGACCTTCCAAAATTTTTCCCATAACGGGAACGAGC42360.14211588953609697No Hit
TCTGGCCTTGAAACAACAGATGCAGTCATGGCTCTTCTCTTTTCCCAGCA42310.1419481417911299No Hit
TGCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCT41920.1406397093803868No Hit
TAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCCCTT40080.13446659236559882No Hit
TCATTTGTCGTCGCTGTTACACCCCGTTGGGCAGCTACATGATTTTTGGC39450.1323529707790138No Hit
TGTCGCTGTTACACCCCGTTGGGCAGCTACATGATTTTTGGCATTGTTCA38700.12983675460450783No Hit
TCGTCGCTGTTACACCCCGTTGGGCAGCTACATGATTTTTGGCATTGTTC36200.12144936735615462No Hit
TGGCCTTGAAACAACAGATGCAGTCATGGCTCTTCTCTTTTCCCAGCAAA36160.121315169160181No Hit
TCTCGATCCAGCACGGGAAAGAGCAATCGTCATAACTATGGTTTAGATTC35490.11906734937762233No Hit
TGCCTCCATCACGGGTCACCTTTGCTGACGCTGGATAAAACTCCCCTAAC35290.11839635839775405No Hit
TCTTGCTGTCTCTAGCAGCTTCTAAAGCAGCGACGGCAGTAGAAGAAGAG34600.11608143951720856No Hit
TAGCCGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCT33750.11322972785276848No Hit
TCCCTTTAGCAAACCTCCATTTATATCCTGCGAGAAGAGCTCCTTCTCCT33030.11081416032524274No Hit
TATTTGTCGTCGCTGTTACACCCCGTTGGGCAGCTACATGATTTTTGGCA32260.10823084505274996No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGCC4700.097.5764541
TCGGGTC8850.076.160091
TCGGCCG4750.073.143441
TCGCCGG6650.071.053621
TCGGGCT9050.068.334561
TGGCCCC14350.068.2756961
TCCCGCA14050.067.755291
TCCCGTT19200.065.867191
TCGCGGC3450.064.451021
TCGGGAC5400.064.3391341
TGGGCCC17250.064.04821
TCCCGGA7400.061.9742361
TGTCGCT13200.058.4316251
CGCCGGG3750.057.4419752
TCGGCTC5100.057.2239841
TCCGGCC7400.056.3402141
TGGCCCG7150.055.394641
TCGCCTC11300.054.1131941
TATCGCG8400.053.769131
TCGCCCG5350.053.2511521
Produced by FastQC (version 0.11.2)