FastQCFastQC Report
Sun 15 Jan 2017
HH75LAFXX_n01_tpq1ss_7_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH75LAFXX_n01_tpq1ss_7_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2580602
Sequences flagged as poor quality0
Sequence length145
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAG496701.924744691354963No Hit
TATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAAGTTAT275121.0661078306534677No Hit
TCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGT259161.0042617962785427No Hit
TGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAG241380.9353631439485826No Hit
TGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT239140.9266829987731545No Hit
TCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTC232400.9005650619506611No Hit
TGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC229090.887738597428042No Hit
TCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTC224260.8690220343935252No Hit
TGCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATT178390.691272811537773No Hit
TCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC150800.5843597734172103No Hit
TGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT131670.5102297835931306No Hit
TGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAAGT126070.48852942065456045No Hit
TCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGT124590.4827943247350812No Hit
TCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG117800.4564826346720649No Hit
TCTCTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTACTTGTTCGCT114440.4434624169089228No Hit
TCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTT113960.4416023857999025No Hit
TCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGC112830.4372235625640839No Hit
TTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCT100920.3910715406715177No Hit
TCGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTAAGGTA97730.3787100839261537No Hit
TTGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAA95760.3710762062495495No Hit
TGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAA93730.36320982468431784No Hit
TGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGGTTCGCC84880.3289155011117561No Hit
TCTCTAGAATTACCACAGTTATACCATGTAGTAAAGGAACTATCAAATAA80400.3115552107609No Hit
TGGCCCAGTGAAATGCGAGATTCCCCTACCCACAAGGAGCAGAGGGCACA78430.3039213330842958No Hit
TAAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCCCT76740.2973724735546202No Hit
TGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCA76120.2949699333721357No Hit
TACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTTGATAG71930.2787334118163126No Hit
TCGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGGTTCGC71880.2785396585757897No Hit
TCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTTTT70740.2741220846918665No Hit
TCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAAT66230.25664554239669657No Hit
TCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCT65320.25311923341917897No Hit
TATGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAA65220.25273172693813306No Hit
TCTCCGGAATCGAACCCTTATTCCCCGTTACCCGTTGAAACCATGGTAGG63310.24533035315015644No Hit
TATGAACAACAATTGTTGTAGTCGCAACTACGGTAATTGGTCCTAAAAAA59540.23072135881472616No Hit
TAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAAG54990.2130898139271379No Hit
TCCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTTGATAGG52470.2033246506047814No Hit
TCCCTGAATTAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATG50780.1967757910751057No Hit
TCTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTACTTGTTCGCTAT50100.19414074700399364No Hit
TTGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAA49920.19344323533811103No Hit
TAGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTC47510.18410432914490493No Hit
TGCATGTATTAGCTCTAGAATTACCACAGTTATACCATGTAGTAAAGGAA46420.1798805085015047No Hit
TAGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC46320.1794930020204588No Hit
TATTTGATGGAAGCAAGTACTTTTTAGATACCCAGTAAAAGTCTCCATAT45710.17712921248607882No Hit
TCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATTTGAGC45000.17437791647065295No Hit
TCGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAA44190.17123911397418123No Hit
TCCTGCCACCAAAACTGATGCTGGCCCAGTGAAATGCGAGATTCCCCTAC43920.1701928464753573No Hit
TCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGGTTCGCCA41630.1613189480594063No Hit
TGGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAA41160.15949766759849057No Hit
TTGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTC41150.159458916950386No Hit
TCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAG40340.15632011445391422No Hit
TAACTTCGGCAGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCCCC40290.1561263612133913No Hit
TCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTTTTC40040.15515759501077656No Hit
TGCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAA38220.14810497705574124No Hit
TCTTCGGCAGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCCCCTA37500.1453149303922108No Hit
TGTACTCATTCCAATTACAAGACCCGAATGGGCCCTGTATCGTTATTTAT36530.14155611752606564No Hit
TCTCTAAAGAAGAGCGGTCACCGAGAGTACTAACGATGGGTTCGTAAGCG36490.14140111493364727No Hit
TCGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCC36140.14004484224998665No Hit
TCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCACATTCT35380.13709979299403782No Hit
TCTCGTTAAGGTATTTACATTGTACTCATTCCAATTACAAGACCCGAATG34530.13380598790514772No Hit
TGTCACTACCTCCCTGAATTAGGATTGGGTAATTTGCGCGCCTGCTGCCT34180.13244971522148707No Hit
TGCGGCTGCTGGCACCAGACTTGCCCTCCAATTGTTCCTCGTTAAGGTAT34020.13182970485181364No Hit
TAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCCCTTG33480.1297371698541658No Hit
TAGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCC32530.1260558582842298No Hit
TGGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTC30390.11776321958984763No Hit
TGCCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTTGATAG30130.1167557027391283No Hit
TGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAA30090.11660070014670997No Hit
TTTCGGCAGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCCCCTAT29910.11590318848082734No Hit
TAGCATGTATTAGCTCTAGAATTACCACAGTTATACCATGTAGTAAAGGA29430.11404315737180704No Hit
TCCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCT28670.11109810811585823No Hit
TGTGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT28080.10881181987768747No Hit
TATAAGGTTATGTTGGCCACTCATCACATTAAAAGACTCCCAATATTCTC27790.10768805108265435No Hit
TCCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAA27720.10741679654592223No Hit
TCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACG27550.10675803552814421No Hit
TACTCGATCCAGCACGGGAAAGAGCAATCGTCATAACTATGGTTTAGATT27510.10660303293572584No Hit
TGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGA26940.10439424599376423No Hit
TGGGCCCTGTATCGTTATTTATTGTCACTACCTCCCTGAATTAGGATTGG26870.10412299145703212No Hit
TCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATTTGA26860.10408424080892752No Hit
TAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAAGTTA26780.10377423562409083No Hit
TCACGTACTTTTTCACTCTCTTTTCAAAGTTCTTTTCATCTTTCCATCAC26520.10276671877337147No Hit
TCCCGGTTGGTTTCTTTTCCTCCGCTTATTGATATGCTTAAGTTCAGCGG26520.10276671877337147No Hit
TGTCCTAGAAACCAACAAAATAGAACCAAACGTCCTATTCTATTATTCCA26080.10106169025676955No Hit
TCTGCCACCAAAACTGATGCTGGCCCAGTGAAATGCGAGATTCCCCTACC25960.10059668247951448No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCG5350.0122.091981
TCGCCTG31100.0121.9959261
TCGCGGC15200.0120.233421
TCCCGTT44700.0115.81421
TGCCTGC45300.0111.5191041
TGCGCCT33650.0111.3055341
TGCCCCG6900.0108.764641
TCGGGCG1600.0108.575811
TCGCCCT12400.099.1892551
CGCGCGC5750.099.096672
TAACGGC5750.096.679681
TGGCCCT14950.094.355651
TAGCGCC8800.092.3881451
TAGCGTG4300.092.1126861
TCGGGCC3950.086.200941
TGCGGCT8300.084.558321
TGGCCCC11150.084.134081
TGTGCCC8950.083.852071
TGCCCGT10150.082.838531
TGTGCCT6600.081.069941
Produced by FastQC (version 0.11.2)