FastQCFastQC Report
Sun 15 Jan 2017
HH75LAFXX_n01_tpq1ss_6_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH75LAFXX_n01_tpq1ss_6_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2625437
Sequences flagged as poor quality0
Sequence length145
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTTGATGGAAGCAAGTACTTTTTAGATACCCAGTAAAAGTCTCCATAT737632.8095513242176446No Hit
TCAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTTGGCGT139830.532597049557845No Hit
TATGAACAACAATTGTTGTAGTCGCAACTACGGTAATTGGTCCTAAAAAA124270.473330725513505No Hit
TCTTATAAGGGTTCGTGATGCTCCTGAATCGAGAAGGAGGTGTCCAGGGA110350.4203109806100851No Hit
TGCCCTGGCTGTTTTCGATTTCGAATTATTTGTTTTTTGAGGATTCCGAG89090.3393339851613274No Hit
TCCTGGCTGTTTTCGATTTCGAATTATTTGTTTTTTGAGGATTCCGAGCT86840.3307639832911626No Hit
TATCAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTTGGC78110.2975123760349229No Hit
TGTGCTAGATCTCTTCTTTGATGGAAGAATATTAGATTCAGATATGTTGG75760.28856148519275077No Hit
TCCCTGTTGTTCTTCATAAATAGCATGGATATCTAAGAACAGTTCAGCGA70230.2674983250407456No Hit
TGTTATTAGATGTGGATACATTGTGAGCCCTGGCTGTTTTCGATTTCGAA68910.26247059061024886No Hit
TATGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTG68820.2621277905354423No Hit
TATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAACTACAAAT67520.2571762338993471No Hit
TGCAAATATTTGAAAAGTGTTATACAAGAAGGTGAGTTCATCATCAGTGA65730.25035832130041585No Hit
TCATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAACTACAAA63350.2412931637666415No Hit
TCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAACTACAAATGC62960.23980769677581296No Hit
TCTGGCTGTTTTCGATTTCGAATTATTTGTTTTTTGAGGATTCCGAGCTA60040.228685738793199No Hit
TCAAATATTTGAAAAGTGTTATACAAGAAGGTGAGTTCATCATCAGTGAT58760.22381035995150522No Hit
TAGTATATGTTGAGCAAGTGTGTTGATGTAGTATAGTAAGTCAAATCTAA58410.22247724854947956No Hit
TAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTTGGCGTT58080.22122031494185537No Hit
TGCCTCGATATATTTTTCTTTTTCTTTAATATCTTTATTATAGGTGATTG57840.22030618140903782No Hit
TATCTTATAAGGGTTCGTGATGCTCCTGAATCGAGAAGGAGGTGTCCAGG57570.21927778118461805No Hit
TTGCTAGATCTCTTCTTTGATGGAAGAATATTAGATTCAGATATGTTGGG57290.2182112920629975No Hit
TGCCTTCCATTGCCTCCTTTTTTTCTCTTCCAGAACTCTCTCAGCAGTAT57100.21748760301618358No Hit
TGATATTTGATGGAAGCAAGTACTTTTTAGATACCCAGTAAAAGTCTCCA56980.2170305362497748No Hit
TTGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTGG56030.21341209101570519No Hit
TTGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAA54390.20716551187478505No Hit
TATAATGGTTCACCGTGTTCCTTGAAGTAAGCTTCATCAGCTTCCAACAT54120.20613711165036525No Hit
TGATGGAAGCAAGTACTTTTTAGATACCCAGTAAAAGTCTCCATATTTTA53430.2035089777435147No Hit
TCGTGCTTATTGTTCAATTGAATCGGTTCAGTAGTTGATTTACTGATGGA51500.1961578205837733No Hit
TGTCCGTATTGGTTTGATTGATTTTACTGCTTTTACAGCTGCAATCAGGT47970.18271243987191466No Hit
TCATCGATTGAAGGTGAACGGTGTATAATCCTTTTCTCAGTCTCTTGGTC47420.18061755052587436No Hit
TGCTAGATCTCTTCTTTGATGGAAGAATATTAGATTCAGATATGTTGGGG45590.17364728233814028No Hit
TAGCAGCTGGAGCACCGGTAATGATAACCTTCTTGTTTTCAGAAATTAAT43290.16488683598197176No Hit
TAGCATCGATTGAAGGTGAACGGTGTATAATCCTTTTCTCAGTCTCTTGG41820.15928776809346407No Hit
TCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCACATTCT41450.15787847889703693No Hit
TGCCGTCAGATCGTTCTAAGACGTTTTTGGTAAAGCATGCTGTGATATCT40590.15460283373777392No Hit
TGTAGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAA40070.15262221108333585No Hit
TGGGTCAAGATCATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAA39250.14949892151287578No Hit
TTAATGGTTCACCGTGTTCCTTGAAGTAAGCTTCATCAGCTTCCAACATA37540.14298572009155047No Hit
TGTATATGTTGAGCAAGTGTGTTGATGTAGTATAGTAAGTCAAATCTAAA36860.14039567508190065No Hit
TGCAAGTAAAGGAACATTTAATTTATGCATACCACCCGAACCGGTACTCT36700.13978625272668893No Hit
TCGGGTCAAGATCATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAA35770.13624398528702078No Hit
TCTCGGTATTCTGTTCAGAAACAGTAGTTGGCGTTTTGATAGGAACAATA35100.13369202917457168No Hit
TCTCTGGAACAGCTGATGAAGCAGGTGTTGTTGTCTGTTGAGAGTTAGCC34730.1322827399781446No Hit
TTGGAAGATCAGCGATAATAGATTCCTCTGTATTCTCCTTAGGACAGGTA34200.13026402842650575No Hit
TAATAGTATATGTTGAGCAAGTGTGTTGATGTAGTATAGTAAGTCAAATC33600.1279786945944618No Hit
TAGCAGATCTTATAAGGGTTCGTGATGCTCCTGAATCGAGAAGGAGGTGT32350.12321758244437021No Hit
TTGAGAAAAGAAATTATACATTTCAGAAACGTCAATAGGGTATCATCATC32060.12211300442554897No Hit
TGTCAAGATCATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAA30990.11803749242507058No Hit
TGTAAAGGAACATTTAATTTATGCATACCACCCGAACCGGTACTCTCGAT30780.11723762558385518No Hit
TGTGCACTTTTTGGTAGGTTGTGAACTGGACCAAATATGTCAGTATGTAG30160.11487611395740976No Hit
TCCTCGGTATTCTGTTCAGAAACAGTAGTTGGCGTTTTGATAGGAACAAT30030.11438095829380023No Hit
TGCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCT29930.1140000693217929No Hit
TACAGAAAGCTAAAAAAAAGAAAATAGAAAACAACCAGAAGAAAACAAAA28880.11000073511571598No Hit
TGTGCTTATTGTTCAATTGAATCGGTTCAGTAGTTGATTTACTGATGGAA28450.10836291253608447No Hit
TAATAATGGTTCACCGTGTTCCTTGAAGTAAGCTTCATCAGCTTCCAACA28400.10817246805008081No Hit
TGTCAGTATGTAGGTATTGAAAGGGTTCGTATGAATTTTGGTATTTTAGT28080.10695362333965736No Hit
TGTAGAGCGTAGCCTGGGATGCCACGAGGATGTATTTTGGAGTTAGGGTT28010.10668700105925222No Hit
TATTTGAAAAGTGTTATACAAGAAGGTGAGTTCATCATCAGTGATCTGAC27520.10482064509641632No Hit
TATGAAGCCTTGTTGGCAATGATGCTAGACAGGTTTAACAACATAGAGTC26670.10158308883435406No Hit
TGCGGAAACTGTGATTGTGGCCCAGGTGGAAAGTACATAGGCGACATTTG26350.10036424412393061No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGTC6650.088.824441
TCGGGCC3250.085.5286941
GGAAGCA133300.080.959589
TATCGCG14000.074.9521561
TTGCTAG16300.071.1973951
TCCCTGG7900.069.4920651
TGCGCCC5500.069.4920651
TGTGCAC9150.069.112331
TATTTGA171250.066.671811
TGCCCGC8800.066.3333361
TGGCCCG6500.066.284741
TGTCCGC2950.065.958571
TGGGCCC10500.065.5210951
TGTGCTA20900.064.17211
TAGCGAT16550.063.403641
TGCCCGT8200.062.712351
TCGGCCG3600.061.770731
TGCCCCT7300.060.924551
TGGCCCT11550.059.5646321
TGCCCCG2500.058.3733371
Produced by FastQC (version 0.11.2)