FastQCFastQC Report
Sun 15 Jan 2017
HH75LAFXX_n01_tpq1ss_5_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH75LAFXX_n01_tpq1ss_5_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3024616
Sequences flagged as poor quality0
Sequence length145
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTCGCTG33300911.00995961140191No Hit
TTTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTCGC1607085.313335643268434No Hit
TATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTCG1230844.069409141524081No Hit
TGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTC1006263.3269016628887766No Hit
TCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTT867962.869653536184428No Hit
TTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTCGCT775182.5629038529188497No Hit
TGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTA637102.106383091275058No Hit
TCGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTT473561.5656863548959603No Hit
TGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG405731.3414264819071247No Hit
TATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAA380031.2564570180148489No Hit
TGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATC328611.0864519661338827No Hit
TGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTCGCTGC323621.0699540040785342No Hit
TGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGC283020.9357220883576626No Hit
TGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTC276040.9126447787090989No Hit
TCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTAT226400.7485247714089988No Hit
TGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACG219690.726340137062027No Hit
TCACGGAATTCTGCAATTCACATTACGTATCGCATTTCGCTGCGTTCTTC218510.7224388153735879No Hit
TCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTC211980.7008492978943442No Hit
TCCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTCGCT179530.5935629514622682No Hit
TACGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTCGCT179000.5918106629072913No Hit
TCCCGGTTGGTTTCTTTTCCTCCGCTTATTGATATGCTTAAGTTCAGCGG156570.5176524887787409No Hit
TCGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGT138980.45949634598243216No Hit
TCGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAG122210.40405129113910654No Hit
TGCCGCTTCACTCGCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCT115920.38325526281683364No Hit
TAGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGT108910.36007876702364866No Hit
TGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAG105280.34807724352446723No Hit
TCTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTCGC101680.3361749061699072No Hit
TGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTC100970.3338275007472023No Hit
TGTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTCGC97540.32248721821216314No Hit
TTGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTCGCTGCGT92400.30549332543370794No Hit
TCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAG86850.2871438886787612No Hit
TGCTACTACCACCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACG80810.267174411561666No Hit
TACGGAATTCTGCAATTCACATTACGTATCGCATTTCGCTGCGTTCTTCA78330.2589750236063024No Hit
TGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT74830.24740330673381347No Hit
TTGCCGCTTCACTCGCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCC74220.2453865217931797No Hit
TCCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAA72290.23900554648920722No Hit
TCGGACGGGAAACGGTGCTTTCTGGTAGATATGGCATCTGAGAGATCGGA71480.23632752058443124No Hit
TGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCA70330.23252538504061343No Hit
TCTGCAATTCACATTACGTATCGCATTTCGCTGCGTTCTTCATCGATGCG67060.22171409527688807No Hit
TGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC65510.21658947780478582No Hit
TCCGGTTGGTTTCTTTTCCTCCGCTTATTGATATGCTTAAGTTCAGCGGG61770.20422427177532618No Hit
TGTCGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCC60730.2007858187617866No Hit
TTGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGC60420.19976089526736618No Hit
TGCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTCGCT58920.19480158803629946No Hit
TCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTC57270.18934635008212614No Hit
TATCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTCGC56980.1883875506841199No Hit
TACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGT56250.18597402116500078No Hit
TACGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGA54040.1786673085112292No Hit
TGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTCGCTGCGTT50240.16610373019252692No Hit
TTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGT50160.16583923380687002No Hit
TCGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGAC49110.16236771874512335No Hit
TGCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCTCCGCTTATTGAT48130.15912763802082644No Hit
TGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTA45940.1518870494634691No Hit
TCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGATCTGAGAGATCG45790.1513911187403624No Hit
TTACCAGCTTAACTACAGTTGATCGGACGGGAAACGGTGCTTTCTGGTAG45690.15106049825829132No Hit
TCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACG45640.15089518801725574No Hit
TCGGAATTCTGCAATTCACATTACGTATCGCATTTCGCTGCGTTCTTCAT44750.14795266572682284No Hit
TGGCGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG43770.14471258500252596No Hit
TCGCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCTCCGCTTATTGA43060.14236517957982106No Hit
TGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT41280.13648013499895525No Hit
TCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGT40260.13310780608182987No Hit
TGCCGACTTCCCTTATCTACATTATTCTATCAACTAGAGGCTGTTCACCT39840.13171920005713122No Hit
TCCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGA39570.13082652475553921No Hit
TCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC39410.13029753198422542No Hit
TACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGC39220.12966935306829033No Hit
TGGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATT38990.12890892595952677No Hit
TCGTTAATTGTAGCAAGCGACGGTCTACAAGAGACCTACCAAGGCCGTCT38350.12679295487427164No Hit
TCAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCA38240.12642927234399343No Hit
TCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCG37880.1252390386085374No Hit
TCGGACGGGAAACGGTGCTTTCTGGTAGATATGATCTGAGAGATCGGAAG37460.12385043258383874No Hit
TCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCC37290.12328837776431785No Hit
TTGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAA36490.12064341390774895No Hit
TCGGACGGGAAACGGTGCTTTCTGGTAGATATGGATCTGAGAGATCGGAA35660.11789926390655872No Hit
TCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCA34970.11561798258026804No Hit
TGCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATT34960.11558492053206093No Hit
TGTCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCT34680.11465918318226181No Hit
TGTCACCCACTACACTACTCGGTCAGGCTCTTACCAGCTTAACTACAGTT34260.11327057715756314No Hit
TAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTT34050.11257627414521379No Hit
TATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTCGCTGCG32610.10781533920338979No Hit
TGTTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTCG32260.1066581675161409No Hit
TCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTATCTGAGAGATC32100.10612917474482711No Hit
TCGGACGGGAAACGGTGCTTTCTGGTAGATCTGAGAGATCGGAAGAGCAC31680.10474056872012844No Hit
TGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACA30810.10186417052610976No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTATAC31750.0135.907531
TAGGGCT3650.0135.164051
TGATTCG110500.0135.009841
TCGCTCA52400.0133.402011
TCCCACG26000.0133.359181
TACGGGC8300.0133.111251
TCGGCGC4650.0128.511261
TCCGATG22100.0127.652541
TCGCGCG4100.0127.1080551
TCGGGCG8800.0125.5480961
TTTCGAT181500.0125.227471
TGTTCAA37550.0124.907791
TAGGCGC1950.0124.7179951
CCGATGA22650.0124.55282
TGTCCCC8350.0123.992531
TGGCGCA32000.0123.988621
TACGATG17850.0123.789721
TCGTTCA28050.0122.126461
TGGGCGC4350.0121.4003751
TACGGAC2950.0120.1278841
Produced by FastQC (version 0.11.2)