FastQCFastQC Report
Sun 15 Jan 2017
HH75LAFXX_n01_tpq1ss_4_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH75LAFXX_n01_tpq1ss_4_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3989704
Sequences flagged as poor quality0
Sequence length145
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT529151.3262888675450608No Hit
TATGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTG457171.1458744809138723No Hit
TATTTGATGGAAGCAAGTACTTTTTAGATACCCAGTAAAAGTCTCCATAT437141.09567025523698No Hit
TTGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTGG433441.0863963842931705No Hit
TGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC422441.058825416622386No Hit
TGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAAGT373560.936310062099845No Hit
TCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAG302500.7582016109465765No Hit
TGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT272580.6832085788820423No Hit
TCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTC265810.6662399015064777No Hit
TGTCAGTCAGGACCATAGCTACTGGCAATTATCGAGATGCTGCTGAAAGA234010.5865347404218458No Hit
TCTTATAAGGGTTCGTGATGCTCCTGAATCGAGAAGGAGGTGTCCAGGGA197610.49529990194761314No Hit
TTCAGTCAGGACCATAGCTACTGGCAATTATCGAGATGCTGCTGAAAGAT172830.43319003114015475No Hit
TATGAACAACAATTGTTGTAGTCGCAACTACGGTAATTGGTCCTAAAAAA166330.4168980956983275No Hit
TCAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTTGGCGT162540.407398644109939No Hit
TCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC154420.38704625706568707No Hit
TCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCACATTCT146960.36834812808168227No Hit
TGCCTTCCATTGCCTCCTTTTTTTCTCTTCCAGAACTCTCTCAGCAGTAT144240.36153057971217917No Hit
TCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGT139140.34874767651936084No Hit
TGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTGGG138100.34614096684866846No Hit
TATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAAGTTAT130880.32804438625020804No Hit
TGTAGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAA107790.2701704186576247No Hit
TAGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAAGA104940.2630270315792851No Hit
TGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAG104640.2622750960973546No Hit
TCATCGATTGAAGGTGAACGGTGTATAATCCTTTTCTCAGTCTCTTGGTC103850.260294999328271No Hit
TCCTAAGCGCAGCATATCATGAAAGAGCGGTGAGACATGCTTGGAAGGGG103320.25896657997686046No Hit
TGTGCTAGATCTCTTCTTTGATGGAAGAATATTAGATTCAGATATGTTGG101250.2537782251515401No Hit
TCTAAGCGCAGCATATCATGAAAGAGCGGTGAGACATGCTTGGAAGGGGA90460.22673361231810682No Hit
TAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTTGGCGTT88060.2207181284626629No Hit
TTGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAA86080.21575535428192166No Hit
TCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG82550.20690758011120627No Hit
TCTCCCGTAACACTATCTCTGATACCCTACCCTTTTCTTGCAAACCGCCA79500.19926290271157962No Hit
TGTTATTAGATGTGGATACATTGTGAGCCCTGGCTGTTTTCGATTTCGAA78420.19655593497662985No Hit
TGCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCT78210.1960295801392785No Hit
TATCAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTTGGC77820.19505206401276887No Hit
TTGCTAGATCTCTTCTTTGATGGAAGAATATTAGATTCAGATATGTTGGG77750.19487661240031842No Hit
TATTTCTTTTTTGAGCATCAACTACGTTTATGTCAGGATTAGATGATGCT77230.19357325756497223No Hit
TTAGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAAG75150.1883598382235875No Hit
TAGCATCGATTGAAGGTGAACGGTGTATAATCCTTTTCTCAGTCTCTTGG73410.1839986124283907No Hit
TATCTTATAAGGGTTCGTGATGCTCCTGAATCGAGAAGGAGGTGTCCAGG72130.1807903543721539No Hit
TGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAA71570.17938674147255035No Hit
TCAGTCAGGACCATAGCTACTGGCAATTATCGAGATGCTGCTGAAAGATT70300.17620354793237794No Hit
TTGCTACTTTCACGCACAGTAGGGTTGAGTCTGATGCACCACTGACATTG65480.16412245118936142No Hit
TCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCTC64840.16251832216124304No Hit
TAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAGGAG64690.1621423544202778No Hit
TAGTGAGGGCACTATATGGGTGTGATTTTTCATCACATACAATGGCTGAT60730.15221680605879534No Hit
TCGTGCTTATTGTTCAATTGAATCGGTTCAGTAGTTGATTTACTGATGGA59600.14938451574352385No Hit
TGACGAACTTCCACCGGATATAGAATAGGTGTCGAAGACGATGAGGACTT58570.1468028705888958No Hit
TTGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTGGGCC57740.14472251575555478No Hit
TCTTTAAATGGGTCAGGGAATTCGGTAGGAGATTCTGGAGGTAGATCAGG56780.14231632221337723No Hit
TCTGTGATTGTGGCCCAGGTGGAAAGTACATAGGCGACATTTGATAAGGT56630.141940354472412No Hit
TCAAATATTTGAAAAGTGTTATACAAGAAGGTGAGTTCATCATCAGTGAT55030.13793003190211603No Hit
TGCAAATATTTGAAAAGTGTTATACAAGAAGGTGAGTTCATCATCAGTGA54900.1376041931932795No Hit
TGCTAGATCTCTTCTTTGATGGAAGAATATTAGATTCAGATATGTTGGGG52960.13274167707679568No Hit
TGTTGGCGATTATACTGCATTCCTGAAAACTAACTTTTCCGAGATAGCTG51940.130185096438232No Hit
TGCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATT51410.1288566770868215No Hit
TCGATTATACTGCATTCCTGAAAACTAACTTTTCCGAGATAGCTGATGCT50940.12767864483179706No Hit
TACAGAAAGCTAAAAAAAAGAAAATAGAAAACAACCAGAAGAAAACAAAA49280.12351793516511501No Hit
TAGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTC49200.1233174190366002No Hit
TGCCCTGGCTGTTTTCGATTTCGAATTATTTGTTTTTTGAGGATTCCGAG46220.11584819324942401No Hit
TATTAGATGTGGATACATTGTGAGCCCTGGCTGTTTTCGATTTCGAATTA45780.11474535454259263No Hit
TTGAGAAAAGAAATTATACATTTCAGAAACGTCAATAGGGTATCATCATC45370.1137177093839543No Hit
TGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAG45050.11291564486989511No Hit
TGAAGAAATTAAAAGAATTTCTACTAAATCCCAATTGTTATATATTTGTT43930.11010841907068795No Hit
TGTAAAGGAACATTTAATTTATGCATACCACCCGAACCGGTACTCTCGAT43460.10893038681566351No Hit
TGTGGACACGAAACTCGGTAGATAGAATGGTCGCTCACCAAACTAACACT42940.10762703198031734No Hit
TGGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTC42590.1067497739180651No Hit
TATTCGGTAGGAGATTCTGGAGGTAGATCAGGGAGTGGGAGATCAGCGAT42330.10609809650039201No Hit
TCTCTGGAACAGCTGATGAAGCAGGTGTTGTTGTCTGTTGAGAGTTAGCC41060.1029149029602196No Hit
TCAGAAAGCTAAAAAAAAGAAAATAGAAAACAACCAGAAGAAAACAAAAA40940.10261412876744741No Hit
TATGAAATTAGCTGGCGTATTTGATCTGGATGACGTTCAAGACTCGGTAC40820.1023133545746752No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCCT69300.0116.819381
TGCCTGC63200.0109.1828461
TCCCGTT25500.0102.191161
TCGCCTG24350.0101.88061
GTCAGTC37650.093.022452
TCGCGCG4850.087.399771
TCCTAAG17750.084.562461
TGCCCCG6350.078.7917941
GGCGCCT8950.078.418862
TATGATG86800.078.056151
TAGCGAT20500.077.964371
TGCGCGC59550.070.948631
TCCCGCA13150.069.754211
TGCCCGT11000.069.489981
TCCCCCG5150.067.466011
TCGGGCT5650.066.415211
TGCGGCT9150.066.072451
TGTAGGA23950.065.573011
TCGGGCC3750.064.857321
TGCCCGC21150.064.72591
Produced by FastQC (version 0.11.2)