FastQCFastQC Report
Sun 15 Jan 2017
HH75LAFXX_n01_tpq1ss_3_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH75LAFXX_n01_tpq1ss_3_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3328304
Sequences flagged as poor quality0
Sequence length145
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTG236140.7094904792350699No Hit
TTGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTGG209170.6284582177589547No Hit
TCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCACATTCT122270.36736427922449394No Hit
TGTCAGTCAGGACCATAGCTACTGGCAATTATCGAGATGCTGCTGAAAGA119600.3593421754743557No Hit
TGACGAACTTCCACCGGATATAGAATAGGTGTCGAAGACGATGAGGACTT116770.3508393464058571No Hit
TCTTATAAGGGTTCGTGATGCTCCTGAATCGAGAAGGAGGTGTCCAGGGA111000.3335031896124873No Hit
TTCAGTCAGGACCATAGCTACTGGCAATTATCGAGATGCTGCTGAAAGAT96870.2910491349347896No Hit
TCAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTTGGCGT96710.29056840961642927No Hit
TAGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAAGA93920.28218576187752076No Hit
TAGTGAGGGCACTATATGGGTGTGATTTTTCATCACATACAATGGCTGAT91540.27503497276691075No Hit
TATTTGATGGAAGCAAGTACTTTTTAGATACCCAGTAAAAGTCTCCATAT88560.26608146371244934No Hit
TCATCGATTGAAGGTGAACGGTGTATAATCCTTTTCTCAGTCTCTTGGTC88410.2656307837264865No Hit
TTGAGAAAAGAAATTATACATTTCAGAAACGTCAATAGGGTATCATCATC86700.26049303188651035No Hit
TATGAAATTAGCTGGCGTATTTGATCTGGATGACGTTCAAGACTCGGTAC82240.24709281363721583No Hit
TGAAGAAATTAAAAGAATTTCTACTAAATCCCAATTGTTATATATTTGTT77100.23164951278488985No Hit
TTGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAA77010.23137910479331214No Hit
TGCCTTCCATTGCCTCCTTTTTTTCTCTTCCAGAACTCTCTCAGCAGTAT75830.2278337555704046No Hit
TGTGCTAGATCTCTTCTTTGATGGAAGAATATTAGATTCAGATATGTTGG73320.22029237713862676No Hit
TTAGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAAG69790.20968637480230173No Hit
TCCTAAGCGCAGCATATCATGAAAGAGCGGTGAGACATGCTTGGAAGGGG69360.20839442550920828No Hit
TAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAGGAG66600.20010191376749242No Hit
TCTAAGCGCAGCATATCATGAAAGAGCGGTGAGACATGCTTGGAAGGGGA66360.19938082578995187No Hit
TGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT64030.19238026334132938No Hit
TATATGAAATTAGCTGGCGTATTTGATCTGGATGACGTTCAAGACTCGGT62940.18910532210999956No Hit
TGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTGGG62600.18808378080848384No Hit
TAGCATCGATTGAAGGTGAACGGTGTATAATCCTTTTCTCAGTCTCTTGG61890.1859505622082598No Hit
TGTAGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAA58590.17603560251707775No Hit
TCTTTAAATGGGTCAGGGAATTCGGTAGGAGATTCTGGAGGTAGATCAGG57490.1727306159533504No Hit
TGCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCT54680.1642878775496469No Hit
TCGATGGTCTTGGGGCTCAGTACTTAAGTAGGTCTTGTGCTACTCTTGTA50920.15299083256817886No Hit
TAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTTGGCGTT49590.1489948033593085No Hit
TTGCTAGATCTCTTCTTTGATGGAAGAATATTAGATTCAGATATGTTGGG48050.14436782217009023No Hit
TATCTTATAAGGGTTCGTGATGCTCCTGAATCGAGAAGGAGGTGTCCAGG47070.14142337959513313No Hit
TTATGAAATTAGCTGGCGTATTTGATCTGGATGACGTTCAAGACTCGGTA46690.1402816569640273No Hit
TCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCTC45990.1381784836962008No Hit
TAAAGTGAGGGCACTATATGGGTGTGATTTTTCATCACATACAATGGCTG45850.13775784904263552No Hit
TATCAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTTGGC45250.13595512909878424No Hit
TGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC43020.129255019974137No Hit
TCAGTCAGGACCATAGCTACTGGCAATTATCGAGATGCTGCTGAAAGATT42740.1284137506670064No Hit
TTTCTTTAAATGGGTCAGGGAATTCGGTAGGAGATTCTGGAGGTAGATCA42710.12832361466981382No Hit
TACAATGTACAAAGCCTATAAGGGTTTGGCAGTCTCATACTATGCTAACC40470.12159346021276903No Hit
TCAAATATTTGAAAAGTGTTATACAAGAAGGTGAGTTCATCATCAGTGAT39700.11927996961815987No Hit
TCACGGTATGAGCGGAGGCGATGGTCTTGGGGCTCAGTACTTAAGTAGGT39150.1176274763362962No Hit
TCTCTGGAACAGCTGATGAAGCAGGTGTTGTTGTCTGTTGAGAGTTAGCC37960.11405208178099115No Hit
TCTGTGATTGTGGCCCAGGTGGAAAGTACATAGGCGACATTTGATAAGGT37510.1127000418231027No Hit
TTGCTACTTTCACGCACAGTAGGGTTGAGTCTGATGCACCACTGACATTG37300.11206908984275475No Hit
TGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAG37230.1118587725159721No Hit
TATGATATTTGTGAAGGCGGTACGCATTGGTCAACTCAATGCATATTATG37150.11161840985679193No Hit
TGTGAGGGCACTATATGGGTGTGATTTTTCATCACATACAATGGCTGATT37100.11146818319480432No Hit
TATGAGAAAAGAAATTATACATTTCAGAAACGTCAATAGGGTATCATCAT36080.10840355929025713No Hit
TATTCGGTAGGAGATTCTGGAGGTAGATCAGGGAGTGGGAGATCAGCGAT34490.10362635143905123No Hit
TCTCCCGTAACACTATCTCTGATACCCTACCCTTTTCTTGCAAACCGCCA34110.10248462880794543No Hit
TGTAACAGTGAATGTAGCGCCATTCGTTACGAAAGTCGTTGGTTCTGGGC34010.10218417548397021No Hit
TCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAG33470.10056172753450407No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTAAG11100.098.2849961
TCGGGCC3200.078.174131
GTCAGTC23700.076.524892
TGCGCCT17250.073.717831
TGCCCGT10800.066.271071
TATGATG56300.065.538531
TGCCTGC15850.065.323211
TGACGAA31200.061.91571
TGCGGCT11000.061.9075971
TCTAAGC15100.060.7445761
TGCCGGC16150.060.2373771
ACGAACT31450.059.4349863
GACGAAC32450.057.389262
TAGCGTG5800.056.3093341
TAGTGAG24650.056.097911
TCCAAGC34050.055.9186978
TAGCGAT15750.054.7081031
TGCCCCG4900.053.888741
TCGGGCT6000.053.2742231
TCCCGCA12750.052.865471
Produced by FastQC (version 0.11.2)