FastQCFastQC Report
Sun 15 Jan 2017
HH75LAFXX_n01_tpq1ss_2_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH75LAFXX_n01_tpq1ss_2_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2888250
Sequences flagged as poor quality0
Sequence length145
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTG163110.5647364320955596No Hit
TTGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTGG126510.4380160997143599No Hit
TATTTGATGGAAGCAAGTACTTTTTAGATACCCAGTAAAAGTCTCCATAT113630.39342162208950054No Hit
TCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCACATTCT83940.2906258114775383No Hit
TTGAGAAAAGAAATTATACATTTCAGAAACGTCAATAGGGTATCATCATC79540.275391673158487No Hit
TGAAGAAATTAAAAGAATTTCTACTAAATCCCAATTGTTATATATTTGTT75530.26150783346316975No Hit
TGTCAGTCAGGACCATAGCTACTGGCAATTATCGAGATGCTGCTGAAAGA74140.2566952306760149No Hit
TCAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTTGGCGT72920.25247121959664154No Hit
TCTTATAAGGGTTCGTGATGCTCCTGAATCGAGAAGGAGGTGTCCAGGGA65770.22771574482818316No Hit
TATGAACAACAATTGTTGTAGTCGCAACTACGGTAATTGGTCCTAAAAAA65350.22626157707954642No Hit
TGTGCTAGATCTCTTCTTTGATGGAAGAATATTAGATTCAGATATGTTGG60940.2109928157188609No Hit
TGCCTTCCATTGCCTCCTTTTTTTCTCTTCCAGAACTCTCTCAGCAGTAT58660.20309876222626158No Hit
TTTCTTTAAATGGGTCAGGGAATTCGGTAGGAGATTCTGGAGGTAGATCA57380.198667012897083No Hit
TTGATCTTGGAGATCGCGTCTGGGTTGAATTGTGCAGTGGACACGAGAGG56850.19683199169047003No Hit
TTGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAA54890.19004587553016533No Hit
TCCTAAGCGCAGCATATCATGAAAGAGCGGTGAGACATGCTTGGAAGGGG54440.188487838656626No Hit
TCTAAGCGCAGCATATCATGAAAGAGCGGTGAGACATGCTTGGAAGGGGA54250.18783000086557605No Hit
TATAATGGTTCACCGTGTTCCTTGAAGTAAGCTTCATCAGCTTCCAACAT54140.18744914740759974No Hit
TATGAGAAAAGAAATTATACATTTCAGAAACGTCAATAGGGTATCATCAT52780.18274041374534755No Hit
TCATCGATTGAAGGTGAACGGTGTATAATCCTTTTCTCAGTCTCTTGGTC51970.17993594737297672No Hit
TAGCAGCTGGAGCACCGGTAATGATAACCTTCTTGTTTTCAGAAATTAAT49740.17221500908854842No Hit
TGTGATAACAACCTTCTTGGCACCAGCGTCAATGTGCTTTTGAGCAGTGT48360.1674370293430278No Hit
TAGCATCGATTGAAGGTGAACGGTGTATAATCCTTTTCTCAGTCTCTTGG45130.15625378689517874No Hit
TAGTGAGGGCACTATATGGGTGTGATTTTTCATCACATACAATGGCTGAT44570.15431489656366312No Hit
TTCAGTCAGGACCATAGCTACTGGCAATTATCGAGATGCTGCTGAAAGAT43660.15116419977495024No Hit
TATACGGGCCAACCCCCATGGGGCGCTATTTTGAGTGTCAAATTCTGTAG40440.1400155803687354No Hit
TAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAGGAG39350.13624166883060676No Hit
TATCTTATAAGGGTTCGTGATGCTCCTGAATCGAGAAGGAGGTGTCCAGG39260.1359300614558989No Hit
TGCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCT39160.13558383103955682No Hit
TGTTATTAGATGTGGATACATTGTGAGCCCTGGCTGTTTTCGATTTCGAA38590.133610317666407No Hit
TAGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAAGA38020.13163680429325716No Hit
TATCAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTTGGC37500.12983640612827838No Hit
TCTTTAAATGGGTCAGGGAATTCGGTAGGAGATTCTGGAGGTAGATCAGG37390.12945555267030207No Hit
TCAAATATTTGAAAAGTGTTATACAAGAAGGTGAGTTCATCATCAGTGAT36720.12713580888081016No Hit
TCTCCCGTAACACTATCTCTGATACCCTACCCTTTTCTTGCAAACCGCCA36030.12474681900804985No Hit
TTAATGGTTCACCGTGTTCCTTGAAGTAAGCTTCATCAGCTTCCAACATA35920.12436596555007358No Hit
TTGCTAGATCTCTTCTTTGATGGAAGAATATTAGATTCAGATATGTTGGG35090.12149225309443434No Hit
TGTAGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAA33620.11640266597420584No Hit
TAGTGATAACAACCTTCTTGGCACCAGCGTCAATGTGCTTTTGAGCAGTG32960.11411754522634814No Hit
TGTAGCAATGTCAGTGATCATAGCAGTGGTTTCAGAAATGTTGGCAGACA32870.11380593785164028No Hit
TATATGAAATTAGCTGGCGTATTTGATCTGGATGACGTTCAAGACTCGGT32860.11377131481000607No Hit
TTAGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAAG32850.11373669176837184No Hit
TCTCTGGAACAGCTGATGAAGCAGGTGTTGTTGTCTGTTGAGAGTTAGCC31970.1106898641045616No Hit
TCGATGGTCTTGGGGCTCAGTACTTAAGTAGGTCTTGTGCTACTCTTGTA31770.10999740327187744No Hit
TAAGCAGCTGGAGCACCGGTAATGATAACCTTCTTGTTTTCAGAAATTAA31450.10888946593958279No Hit
TCTCTAAAGAAGAGCGGTCACCGAGAGTACTAACGATGGGTTCGTAAGCG29150.10092616636371504No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTAAG11050.077.3494341
TCGGGCC3250.074.834011
TCTAAGC11100.070.1147461
ATACGGG8550.065.831422
TCGGGTC6600.065.277281
TGCCGGC10250.063.7262421
TCGGGCT6900.063.4462241
TGGCCCG6850.062.89491
GTCAGTC16600.059.8607642
TGCCCGC13600.055.1822171
GTCCCGA2750.053.0641172
TGCGGCT11150.052.9734651
TATGAGA17600.051.3268971
CAGTCCA26850.050.7263535
AGGCGAG33550.050.646515136-137
TGTCCCG5900.050.644321
TGGGCCG40800.050.59202248-49
TCGCGCG1800.050.18631
TACACGG40450.049.7368478-79
ACGGGCC11600.049.7212374
Produced by FastQC (version 0.11.2)