FastQCFastQC Report
Sun 15 Jan 2017
HH75LAFXX_n01_tpq1ss_24_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH75LAFXX_n01_tpq1ss_24_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2882777
Sequences flagged as poor quality0
Sequence length145
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAA174620.6057353725244791No Hit
TAAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCCCT106100.3680478927090094No Hit
TGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAG101450.3519176127740717No Hit
TACTCGATCCAGCACGGGAAAGAGCAATCGTCATAACTATGGTTTAGATT88230.30605905347517337No Hit
TATGAACAACAATTGTTGTAGTCGCAACTACGGTAATTGGTCCTAAAAAA79900.27716330468850003No Hit
TAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAGGAG77230.2679014020161809No Hit
TCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAACTACAAATGC67890.23550208705009093No Hit
TGGCCCCAAAAATCAGTTTAACCCAAAAATCCCCAAAGGAATCAAGGGAT67130.2328657402220151No Hit
TGGGCCCCAAAAATCAGTTTAACCCAAAAATCCCCAAAGGAATCAAGGGA66380.23026408216799288No Hit
TCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCACATTCT49820.17281947233518238No Hit
TCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCTC45310.15717483523699544No Hit
TCTCCCGCCTCCGGGCAGACAGCTCTGCCCAGGACAAATTTACGACGGAG40410.14017733595071696No Hit
TAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCCCTTG40210.13948356046964439No Hit
TCTCGATCCAGCACGGGAAAGAGCAATCGTCATAACTATGGTTTAGATTC39920.13847758602208912No Hit
TACCCGTTCCTACCAAGACCTTCCAAAATTTTTCCCATAACGGGAACGAG39510.1370553462858903No Hit
TGCCTCCATCACGGGTCACCTTTGCTGACGCTGGATAAAACTCCCCTAAC39170.1358759279680669No Hit
TCCCGTTCCTACCAAGACCTTCCAAAATTTTTCCCATAACGGGAACGAGC38540.13369053520268825No Hit
TCTGGCCTTGAAACAACAGATGCAGTCATGGCTCTTCTCTTTTCCCAGCA37970.13171327508163136No Hit
TCTCTAAAGAAGAGCGGTCACCGAGAGTACTAACGATGGGTTCGTAAGCG37720.13084605573029062No Hit
TCAGAAATGATCAACACCAGGCGCCCAGCAACGTTCAATCTATCTACGCT37410.1297707037346281No Hit
TTAGGGATTTTATTGGTATCAGGGTTAATCGTGCCAAGAAAAGCGGCATG35770.12408174478983286No Hit
TATGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCA35760.12404705601577924No Hit
TGGCCTTGAAACAACAGATGCAGTCATGGCTCTTCTCTTTTCCCAGCAAA34220.11870498481152028No Hit
TGCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCT33340.11565237269480087No Hit
TCATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAACTACAAA32270.11194067387106253No Hit
AATATCAGCACCAACAGAAACAACCTGATTAGCGGCGTTGACAGATGTAT30480.10573138331546282No Hit
TCTCCCCTTTAGCAAACCTCCATTTATATCCTGCGAGAAGAGCTCCTTCT30370.1053498068008729No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGCC6800.090.945161
TCGGGTC11450.077.678881
TGGCCCC19200.072.381441
TCCCGTT19200.068.038551
TCGCGGC4450.065.5824661
TCGCCGG7000.065.515541
TGGGCCC19450.065.020491
TACTCGA22200.064.478171
TCGGGCT9050.061.424251
ACTCGAT23100.061.364422
TAGACGG12250.057.8578831
TCGCCTC11700.057.6082041
TCGCCCG5200.057.459731
TCCCGCA15200.057.143241
TCGGCGC5300.056.3755841
GGGAGCG5300.055.063573
TGCCCGT10300.053.2952271
TCGGCCG5350.053.2510951
CTCGATC26900.052.6949273
TATCGCG8200.052.5383381
Produced by FastQC (version 0.11.2)