FastQCFastQC Report
Sun 15 Jan 2017
HH75LAFXX_n01_tpq1ss_23_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH75LAFXX_n01_tpq1ss_23_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2141512
Sequences flagged as poor quality0
Sequence length145
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAACTACAAATGC289731.3529226079517649No Hit
TTGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAA187080.8735883805460815No Hit
TCATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAACTACAAA151470.7073039982965307No Hit
TATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAACTACAAAT142440.6651375289982031No Hit
TGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAG117780.5499852440705446No Hit
TCAGAAATGATCAACACCAGGCGCCCAGCAACGTTCAATCTATCTACGCT114360.5340152191535701No Hit
TATGAACAACAATTGTTGTAGTCGCAACTACGGTAATTGGTCCTAAAAAA113920.5319605960648365No Hit
TACTCGATCCAGCACGGGAAAGAGCAATCGTCATAACTATGGTTTAGATT103680.48414391327249157No Hit
TCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAG88030.41106470568458175No Hit
TAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAGGAG83920.39187265819663863No Hit
TGTCAAGATCATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAA83530.3900515150043521No Hit
TGGCCCCAAAAATCAGTTTAACCCAAAAATCCCCAAAGGAATCAAGGGAT80710.37688324884474145No Hit
TCCCGTTCCTACCAAGACCTTCCAAAATTTTTCCCATAACGGGAACGAGC76830.35876520888045454No Hit
TGGGCCCCAAAAATCAGTTTAACCCAAAAATCCCCAAAGGAATCAAGGGA75010.35026654064978385No Hit
TCGGGTCAAGATCATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAA71160.3322885886233652No Hit
TCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCACATTCT68070.3178595310229408No Hit
TACCCGTTCCTACCAAGACCTTCCAAAATTTTTCCCATAACGGGAACGAG66310.3096410386680065No Hit
TCGAAACGTGTGAATCAACAGAATCAACCACAGCATATCATAAACCTGAA65500.3058586643455652No Hit
TATAAGGTTATGTTGGCCACTCATCACATTAAAAGACTCCCAATATTCTC59720.2788683883162924No Hit
TCTCGATCCAGCACGGGAAAGAGCAATCGTCATAACTATGGTTTAGATTC58160.2715838155471461No Hit
TCTCCCCTTTAGCAAACCTCCATTTATATCCTGCGAGAAGAGCTCCTTCT54460.2543063032100684No Hit
TAAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCCCT53200.24842260981960412No Hit
TGTTCCAAAAAATATGGCAAGCCCACAGTAACGGACAGCTTTACCTGTTT51100.23861645416883023No Hit
TCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCTC50990.23810279839664686No Hit
TCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGT50200.2344138160327843No Hit
TGGATGGGTCAAGATCATCGCGCCATAAAATATTGTAATTACTTCCAAAG50120.2340402481984691No Hit
TGGCCTTGAAACAACAGATGCAGTCATGGCTCTTCTCTTTTCCCAGCAAA46280.21610899215133977No Hit
TATGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCA46170.2155953363791564No Hit
TAGGATGGGTCAAGATCATCGCGCCATAAAATATTGTAATTACTTCCAAA45900.21433454493834264No Hit
TCTGGCCTTGAAACAACAGATGCAGTCATGGCTCTTCTCTTTTCCCAGCA45410.21204644195316208No Hit
TCAAGATCATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAAC44540.20798389175498436No Hit
TGGTCAAGATCATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAA42450.1982244320834999No Hit
TGTCAGACTGCCATTTGTACCCACCCATAGAGCCCTATCCCTTCAAAAAA38340.1790323845955568No Hit
TTCAAGATCATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAA37670.17590375398316704No Hit
TGCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCT37620.17567027408672004No Hit
TGCCTCCATCACGGGTCACCTTTGCTGACGCTGGATAAAACTCCCCTAAC36900.17230816357788328No Hit
TGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAGGAGTTT35940.16782534956610096No Hit
TAGGGTCAAGATCATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAA35380.1652103747258946No Hit
TCCTTGAAACAACAGATGCAGTCATGGCTCTTCTCTTTTCCCAGCAAACG35120.16399627926437021No Hit
TGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAG34900.16296896772000344No Hit
TCTCTAAAGAAGAGCGGTCACCGAGAGTACTAACGATGGGTTCGTAAGCG33150.15479717134435855No Hit
TCTCCCGCCTCCGGGCAGACAGCTCTGCCCAGGACAAATTTACGACGGAG32660.152509068359178No Hit
TGGGTCAAGATCATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAA32140.15008087743612925No Hit
TCTGCCATTTGTACCCACCCATAGAGCCCTATCCCTTCAAAAAAGAAGTA31310.14620511115510912No Hit
TGCCTTCCATTGCCTCCTTTTTTTCTCTTCCAGAACTCTCTCAGCAGTAT31280.1460650232172409No Hit
TGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC30760.14363683229419213No Hit
TGCCCCAAAAATCAGTTTAACCCAAAAATCCCCAAAGGAATCAAGGGATT30720.14345004837703454No Hit
TCCCAAAAATCAGTTTAACCCAAAAATCCCCAAAGGAATCAAGGGATTAA30320.14158220920545858No Hit
TCGGCAAAGCAAAATTTTTATTCAAAATTATAGATGGCCGAACCGCTAAG30100.1405548976610918No Hit
TCCCTTTAGCAAACCTCCATTTATATCCTGCGAGAAGAGCTCCTTCTCCT29620.13831349065520063No Hit
TAGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAAGA28720.1341108525191547No Hit
TCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTC28030.13088882994818615No Hit
TAGGGCCCCAAAAATCAGTTTAACCCAAAAATCCCCAAAGGAATCAAGGG28010.13079543798960735No Hit
TGGCCACAATGTGCGAGTAAATCCTGATGGCACCGCCAAATTAAACCGCC27850.13004830232097694No Hit
TTGCCATTTGTACCCACCCATAGAGCCCTATCCCTTCAAAAAAGAAGTAC27570.12874081490087377No Hit
TAGAGAAACATCAAGCGACCAAAGTAAAAGTCAAGAACGACTCCACAAGT26530.12388443305477626No Hit
TGCCCGTTCCTACCAAGACCTTCCAAAATTTTTCCCATAACGGGAACGAG26290.12276372955183067No Hit
TCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTC26060.12168972202817449No Hit
TGGATTCAGTGGCTCTTTTGAAGAGTCAAAGAGTGACGATTCCTATAGAA25090.11716021203710275No Hit
TAGCCGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCT24630.1150121969897904No Hit
TCTCCAAAAAAAACGCCTCTATGACATAGGCGGTTAATAAAACTGGCCTT24260.11328444575608262No Hit
TTGGATTCAGTGGCTCTTTTGAAGAGTCAAAGAGTGACGATTCCTATAGA23680.11057607895729746No Hit
TGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAACTACAAATGCA23660.11048268699871866No Hit
TGTAATTACTTCCAAAGGAAAAAACTACAAATGCAACGGCAAAGCAAAAT23610.11024920710227167No Hit
TGATCATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAACTAC23270.10866154380643209No Hit
TGCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATT23270.10866154380643209No Hit
TGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT22890.10688709659343491No Hit
TCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCTCC22680.10590648102835755No Hit
TATGAAGCCTTGTTGGCAATGATGCTAGACAGGTTTAACAACATAGAGTC22440.10478577752541195No Hit
TCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAACTACAAATGCAA22400.10459899360825435No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG20000.0107.366511
TCCCGTT20900.0104.738091
TCGGGTC14400.0104.2393341
TCGGGCC5200.098.893721
TGGCCCC17000.095.654921
TCGGCGC3000.094.973621
TCGCGGC2750.088.4454961
CGAAACG11600.088.064272
CGTGTGA13050.079.3443767
TCGGGAC4150.078.702791
TACTCGA22650.075.4757161
TGCCCGT8900.073.396991
TGCCTGC9200.072.514321
ACGTGTG14400.071.423256
ACTCGAT24000.070.651112
CCAAGCC19500.070.2072759
TCGCCTC9150.069.8726351
TCGCGCG703.936293E-969.492891
TAGGGCC10100.068.804841
TCGGGCT4250.068.675331
Produced by FastQC (version 0.11.2)