FastQCFastQC Report
Sun 15 Jan 2017
HH75LAFXX_n01_tpq1ss_22_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH75LAFXX_n01_tpq1ss_22_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3110531
Sequences flagged as poor quality0
Sequence length145
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATT405231.3027679196895963No Hit
TTGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAA213890.6876317901991653No Hit
TAAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCCCT165700.5327064735892361No Hit
TATGAACAACAATTGTTGTAGTCGCAACTACGGTAATTGGTCCTAAAAAA130390.4191888780404374No Hit
TCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAG119830.38523969058659113No Hit
TGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAG107790.3465324730729255No Hit
TCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTC97230.31258328561907917No Hit
TGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT93620.30097755013533056No Hit
TGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC91380.2937762073420905No Hit
TCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATTTGAGC90420.290689917573559No Hit
TAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAGGAG81040.26053429462686595No Hit
TCAGAAATGATCAACACCAGGCGCCCAGCAACGTTCAATCTATCTACGCT79650.2560656042328464No Hit
TCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGT76590.24622805559565233No Hit
TCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC76520.24600301363336355No Hit
TCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCTC75370.2423058956814769No Hit
TACTCGATCCAGCACGGGAAAGAGCAATCGTCATAACTATGGTTTAGATT74460.2393803501717231No Hit
TCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATTT74050.23806224724974612No Hit
TAGCCGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCT72230.23221115623023852No Hit
TCTCTGGAACAGCTGATGAAGCAGGTGTTGTTGTCTGTTGAGAGTTAGCC68610.22057327189473436No Hit
TATTTGATGGAAGCAAGTACTTTTTAGATACCCAGTAAAAGTCTCCATAT68300.21957665749031274No Hit
TCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCACATTCT62800.20189478902476776No Hit
TGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAG61320.19713675896494842No Hit
TGCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCT58820.18909954602606435No Hit
TGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT57920.1862061493680661No Hit
TCGGCCCCAAAGTTGCCCTCTCCAAATTACAACTCGGGCACCGAAGGTAC53280.17128908215349725No Hit
TATAAGGTTATGTTGGCCACTCATCACATTAAAAGACTCCCAATATTCTC52770.16964949071396493No Hit
TAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCCCTT51110.1643127813225459No Hit
TCTCCCGCCTCCGGGCAGACAGCTCTGCCCAGGACAAATTTACGACGGAG50610.16270533873476908No Hit
TCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG49280.15842954145128277No Hit
TCTTATGTATAATTCTTCTTTGATGTCTGCATACAAATATGCCGAAGATA48650.15640416379068398No Hit
TAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCCCTTG48210.15498961431344035No Hit
TATTTGCGTGTACTTTCACTTGTATGGACATTATTGATGGTGGGTACGGA47070.15132464521330924No Hit
TCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAACTACAAATGC46200.14852769511057756No Hit
TGGGCCCCAAAAATCAGTTTAACCCAAAAATCCCCAAAGGAATCAAGGGA42750.13743634125491758No Hit
TAACGGCCCCAAAGTTGCCCTCTCCAAATTACAACTCGGGCACCGAAGGT42730.1373720435514065No Hit
TCGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCC40070.12882044898443384No Hit
TGGCCCCAAAAATCAGTTTAACCCAAAAATCCCCAAAGGAATCAAGGGAT39680.12756664376596794No Hit
TTGAAATTGTAGGTCACCAATAGCGTTAATTGGTATATTTCTTTTTTGAG39040.1255091172536136No Hit
TCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACG38530.12386952581408127No Hit
TCTTTAAATGGGTCAGGGAATTCGGTAGGAGATTCTGGAGGTAGATCAGG38180.1227443160026375No Hit
TGTCAAGATCATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAA38060.12235852978157105No Hit
TCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATTTGA37720.12126546882188281No Hit
TCCCGTTCCTACCAAGACCTTCCAAAATTTTTCCCATAACGGGAACGAGC36950.11879000723670652No Hit
TCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCTCC36830.11840422101564009No Hit
TGGGCCCTGTATCGTTATTTATTGTCACTACCTCCCTGAATTAGGATTGG36360.1168932249831299No Hit
TCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTC35440.11393553062162055No Hit
TCCCCAAAGTTGCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAG35140.11297106506895446No Hit
TAAGGTTATGTTGGCCACTCATCACATTAAAAGACTCCCAATATTCTCCA34120.10969188218988976No Hit
TGCCTCCATCACGGGTCACCTTTGCTGACGCTGGATAAAACTCCCCTAAC33670.10824518386089065No Hit
TCGGGTCAAGATCATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAA32980.10602691308975863No Hit
TGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCCCTTGC32010.1029084744694716No Hit
TGGCCCCAAAGTTGCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACC31550.10142962728871695No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGCC5850.0114.025571
TCGCGCG3800.0106.055041
TCCCGTT23550.099.136891
TCGGGAC7100.095.907961
TCGCCTG17100.088.1760251
TGCCTGC21150.084.43251
TGCGCCT20250.081.32241
TCGGGCT5950.078.242871
TGGCCCC16350.076.4965061
CGCGCGC4100.074.568562
TCGGCTC4100.074.568561
TCGCGGC5750.073.713811
TCCCGCA17900.073.366141
TGCCGGG5700.073.14141
CGCGGCG3050.072.905119
TGCCCGT9400.071.701921
TGCCCCG3600.071.414461
TCTCGCG2850.070.703361
TCGGGTC15100.069.9444961
TCGGCCC21050.066.3484651
Produced by FastQC (version 0.11.2)