FastQCFastQC Report
Sun 15 Jan 2017
HH75LAFXX_n01_tpq1ss_21_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH75LAFXX_n01_tpq1ss_21_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3576520
Sequences flagged as poor quality0
Sequence length145
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGAACAACAATTGTTGTAGTCGCAACTACGGTAATTGGTCCTAAAAAA503671.40826837260801No Hit
TCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAACTACAAATGC410781.1485466319215325No Hit
TATTTGATGGAAGCAAGTACTTTTTAGATACCCAGTAAAAGTCTCCATAT334990.9366367306767474No Hit
TTGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTGG246260.6885464082404125No Hit
TTGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAA243090.6796830438526835No Hit
TATGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTG228540.6390010401172089No Hit
TCATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAACTACAAA219970.6150392001163142No Hit
TCAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTTGGCGT160580.448983928511514No Hit
TCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCACATTCT157370.4400087235636876No Hit
TGTCAGTCAGGACCATAGCTACTGGCAATTATCGAGATGCTGCTGAAAGA152060.42516188921074116No Hit
TATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAACTACAAAT145000.40542203035352803No Hit
TTCAGTCAGGACCATAGCTACTGGCAATTATCGAGATGCTGCTGAAAGAT144950.40528222965340616No Hit
TGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAG132620.37080737700334404No Hit
TGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAG124780.34888662722422914No Hit
TCGGGTCAAGATCATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAA120600.33719928869403776No Hit
TAGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAAGA110320.30845626474897386No Hit
TGCCTTCCATTGCCTCCTTTTTTTCTCTTCCAGAACTCTCTCAGCAGTAT108300.3028083164640489No Hit
TCTTATAAGGGTTCGTGATGCTCCTGAATCGAGAAGGAGGTGTCCAGGGA95210.2662084931721338No Hit
TGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTGGG85610.2393667587487278No Hit
TAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTTGGCGTT84160.23531253844519254No Hit
TGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAACTACAAATGCA80700.22563832999675662No Hit
TCTCCCGTAACACTATCTCTGATACCCTACCCTTTTCTTGCAAACCGCCA80420.22485544607607394No Hit
TTGAGAAAAGAAATTATACATTTCAGAAACGTCAATAGGGTATCATCATC78520.2195430194714415No Hit
TGTCAAGATCATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAA75500.21109905718407837No Hit
TGTAGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAA71540.20002684173442342No Hit
TAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAGGAG71140.19890843613344816No Hit
TCTCGTTAATCCATTCATGCGCGTCACTAATTAGATGACGAGGCATTTGG69720.194938096249986No Hit
TGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT68960.19281312560813305No Hit
TCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAG63410.17729524789460147No Hit
TTAGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAAG61930.17315714717099304No Hit
TATGAAGCCTTGTTGGCAATGATGCTAGACAGGTTTAACAACATAGAGTC60820.17005357162828671No Hit
TGTGCTAGATCTCTTCTTTGATGGAAGAATATTAGATTCAGATATGTTGG60570.16935456812767719No Hit
TCAGTCAGGACCATAGCTACTGGCAATTATCGAGATGCTGCTGAAAGATT58220.1627839352219476No Hit
TGCCCTGGCTGTTTTCGATTTCGAATTATTTGTTTTTTGAGGATTCCGAG58090.16242045340163064No Hit
TGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC57470.160686924720119No Hit
TGGTCAAGATCATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAA56340.15752742889736393No Hit
TGGGCCCCAAAAATCAGTTTAACCCAAAAATCCCCAAAGGAATCAAGGGA56300.15741558833726638No Hit
TATCAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTTGGC54440.1522150022927315No Hit
TAGGGTCAAGATCATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAA54120.1513202778119513No Hit
TGGATGGGTCAAGATCATCGCGCCATAAAATATTGTAATTACTTCCAAAG52720.1474058582085379No Hit
TACAGAAAGCTAAAAAAAAGAAAATAGAAAACAACCAGAAGAAAACAAAA52510.1468186952680259No Hit
TGTTGGCGATTATACTGCATTCCTGAAAACTAACTTTTCCGAGATAGCTG52100.14567232952702627No Hit
TGGGTCAAGATCATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAA51410.14374307986534396No Hit
TTGCTAGATCTCTTCTTTGATGGAAGAATATTAGATTCAGATATGTTGGG51200.14315591692483196No Hit
TGTTGACATTGAGAATATAGATAGTTATAAGAAGACAAGGATGATTGCCA50630.14156218894344222No Hit
TCCTAAGCGCAGCATATCATGAAAGAGCGGTGAGACATGCTTGGAAGGGG50060.1399684609620525No Hit
TTGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTGGGCC50010.13982866026193058No Hit
TAGGATGGGTCAAGATCATCGCGCCATAAAATATTGTAATTACTTCCAAA49880.13946517844161366No Hit
TCTGGCCTTGAAACAACAGATGCAGTCATGGCTCTTCTCTTTTCCCAGCA47720.1334257881963473No Hit
TCCTGGCTGTTTTCGATTTCGAATTATTTGTTTTTTGAGGATTCCGAGCT47470.13272678469573776No Hit
TCTCTAAAGAAGAGCGGTCACCGAGAGTACTAACGATGGGTTCGTAAGCG44420.12419894198830149No Hit
TGCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCT44340.12397526086810644No Hit
TCTAAGCGCAGCATATCATGAAAGAGCGGTGAGACATGCTTGGAAGGGGA44290.12383546016798451No Hit
TGAACAACAATTGTTGTAGTCGCAACTACGGTAATTGGTCCTAAAAAATT43770.1223815328867167No Hit
TAGCAGCTGGAGCACCGGTAATGATAACCTTCTTGTTTTCAGAAATTAAT43690.12215785176652165No Hit
TTGAGCAACTTAGTAATGGTTGTTAGCAAAATCAATCCTGCAAAAGCTAA42560.11899835594376657No Hit
TGGCCCCAAAAATCAGTTTAACCCAAAAATCCCCAAAGGAATCAAGGGAT42090.11768422936262064No Hit
TTGCTACTTTCACGCACAGTAGGGTTGAGTCTGATGCACCACTGACATTG41710.11662174404169416No Hit
TGGCCTTGAAACAACAGATGCAGTCATGGCTCTTCTCTTTTCCCAGCAAA41490.11600662096115778No Hit
TCTCCATTTATATCCTGCGAGAAGAGCTCCTTCTCCTCAATGAGAAAATA41220.11525169718049949No Hit
TGTTATTAGATGTGGATACATTGTGAGCCCTGGCTGTTTTCGATTTCGAA40450.11309876639862212No Hit
TCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCTC40310.1127073244382808No Hit
TAACAACAATTGTTGTAGTCGCAACTACGGTAATTGGTCCTAAAAAATTA39870.11147707827720801No Hit
TCCCGTAACACTATCTCTGATACCCTACCCTTTTCTTGCAAACCGCCACA39440.11027479225615962No Hit
TCGATTATACTGCATTCCTGAAAACTAACTTTTCCGAGATAGCTGATGCT39280.10982743001576951No Hit
TGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT39140.10943598805542819No Hit
TAAGGAATATAGATATGTGTATGACAGAAACTACATGCATATACGAGTTT39130.10940802791540381No Hit
TCAGAAAGCTAAAAAAAAGAAAATAGAAAACAACCAGAAGAAAACAAAAA39010.10907250623511122No Hit
TCAAATATTTGAAAAGTGTTATACAAGAAGGTGAGTTCATCATCAGTGAT38790.10845738315457484No Hit
TGATGGAAGCAAGTACTTTTTAGATACCCAGTAAAAGTCTCCATATTTTA38500.10764653909386779No Hit
TCTCTGGAACAGCTGATGAAGCAGGTGTTGTTGTCTGTTGAGAGTTAGCC38270.10700345587330701No Hit
TCGCGCTAATACAACTAAAATGAACATTGGTACGATAGCAGAGTTTTTGA37940.10608077125250244No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGTC20650.099.603181
TACCCGG4350.097.4410861
TATGAAC84250.092.538931
GTCAGTC28000.085.864482
TCGGGCC4050.085.786021
TCCCGTT15650.080.8087841
TAGCGAT17200.079.990481
TGCCTGC19900.079.612871
TATCGCG27150.077.036791
ACAATTG101150.076.806099
TGGCCCC14350.076.023751
TGCGCCT17450.075.2606351
TCGCGCG4500.071.030821
CCAGTCC33600.070.72854
TGCGCCA23800.070.07061
CGGGTCA27650.069.108742
GGAAGCA73300.067.7831659
ACAACAA116250.066.707216
GAACAAC116500.066.624634
TGTTTCA96100.066.594411
Produced by FastQC (version 0.11.2)