FastQCFastQC Report
Sun 15 Jan 2017
HH75LAFXX_n01_tpq1ss_20_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH75LAFXX_n01_tpq1ss_20_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3261791
Sequences flagged as poor quality0
Sequence length145
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTG479911.4713082475241364No Hit
TTGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTGG462631.4183312174201228No Hit
TGTCAGTCAGGACCATAGCTACTGGCAATTATCGAGATGCTGCTGAAAGA394951.210837849512737No Hit
TGACGAACTTCCACCGGATATAGAATAGGTGTCGAAGACGATGAGGACTT338671.0382946056323044No Hit
TTCAGTCAGGACCATAGCTACTGGCAATTATCGAGATGCTGCTGAAAGAT201310.6171762691110497No Hit
TAGTGAGGGCACTATATGGGTGTGATTTTTCATCACATACAATGGCTGAT196760.6032268775038008No Hit
TGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTGGG188720.578577842663739No Hit
TGCCTTCCATTGCCTCCTTTTTTTCTCTTCCAGAACTCTCTCAGCAGTAT146720.44981422782759534No Hit
TATGAAATTAGCTGGCGTATTTGATCTGGATGACGTTCAAGACTCGGTAC111330.34131555332637803No Hit
TGGACGAACTTCCACCGGATATAGAATAGGTGTCGAAGACGATGAGGACT102250.31347808611894507No Hit
TGTGAGGGCACTATATGGGTGTGATTTTTCATCACATACAATGGCTGATT100500.30811293550077246No Hit
TTGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAA99320.3044952910839474No Hit
TCAGTCAGGACCATAGCTACTGGCAATTATCGAGATGCTGCTGAAAGATT96180.29486867797476907No Hit
TCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCACATTCT92330.2830653466147892No Hit
TTGAGAAAAGAAATTATACATTTCAGAAACGTCAATAGGGTATCATCATC88950.2727029414208329No Hit
TCCTAAGCGCAGCATATCATGAAAGAGCGGTGAGACATGCTTGGAAGGGG86130.26405738442469184No Hit
TATGAACAACAATTGTTGTAGTCGCAACTACGGTAATTGGTCCTAAAAAA84410.2587842078171164No Hit
TGTAGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAA83430.25577972347093975No Hit
TCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAACTACAAATGC79170.24271941396613086No Hit
TAAAGTGAGGGCACTATATGGGTGTGATTTTTCATCACATACAATGGCTG79020.24225954391314466No Hit
TAGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAAGA73770.2261640920586267No Hit
TATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAACTACAAAT73180.22435526985021417No Hit
TCTAAGCGCAGCATATCATGAAAGAGCGGTGAGACATGCTTGGAAGGGGA72730.22297565969125552No Hit
TTGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTGGGCC72080.2209828894616485No Hit
TATATGAAATTAGCTGGCGTATTTGATCTGGATGACGTTCAAGACTCGGT65350.20035005308433312No Hit
TGGTCAGTCAGGACCATAGCTACTGGCAATTATCGAGATGCTGCTGAAAG64800.19866386289005025No Hit
TGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAG56870.17435206608884504No Hit
TTATGAAATTAGCTGGCGTATTTGATCTGGATGACGTTCAAGACTCGGTA53710.16466413697260185No Hit
TCATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAACTACAAA53510.16405097690195355No Hit
TAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAGGAG53320.16346847483483767No Hit
TATGATATTTGTGAAGGCGGTACGCATTGGTCAACTCAATGCATATTATG52470.16086254453458237No Hit
TAAGTGAGGGCACTATATGGGTGTGATTTTTCATCACATACAATGGCTGA51790.15877780029437816No Hit
TTGATCTTGGAGATCGCGTCTGGGTTGAATTGTGCAGTGGACACGAGAGG47960.14703578494146313No Hit
TAGCAGCTGGAGCACCGGTAATGATAACCTTCTTGTTTTCAGAAATTAAT47180.14464446066593475No Hit
TATGAGAAAAGAAATTATACATTTCAGAAACGTCAATAGGGTATCATCAT44810.13737851382875235No Hit
TTGCTACTTTCACGCACAGTAGGGTTGAGTCTGATGCACCACTGACATTG42870.13143086114346383No Hit
TGGGCACTATATGGGTGTGATTTTTCATCACATACAATGGCTGATTTTGG42630.13069506905868586No Hit
TATTTGTCGTCGCTGTTACACCCCGTTGGGCAGCTACATGATTTTTGGCA40870.1252992604369808No Hit
TTAGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAAG40690.12474741637339731No Hit
TGCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCT40430.12395030828155452No Hit
TATACGGGCCAACCCCCATGGGGCGCTATTTTGAGTGTCAAATTCTGTAG38050.11665370344083972No Hit
TAAGGAATATAGATATGTGTATGACAGAAACTACATGCATATACGAGTTT37120.1138025091123251No Hit
TACAGAAAGCTAAAAAAAAGAAAATAGAAAACAACCAGAAGAAAACAAAA37090.11371053510172786No Hit
TCGTCGCTGTTACACCCCGTTGGGCAGCTACATGATTTTTGGCATTGTTC36740.11263750497809333No Hit
TTGATATTTGTGAAGGCGGTACGCATTGGTCAACTCAATGCATATTATGT35970.11027683870609736No Hit
TGCCTCCTTTTTTTCTCTTCCAGAACTCTCTCAGCAGTATTCTCACAAAC35950.11021552269903252No Hit
TCTCCCGTAACACTATCTCTGATACCCTACCCTTTTCTTGCAAACCGCCA35640.10926512458952767No Hit
TGTCGCTGTTACACCCCGTTGGGCAGCTACATGATTTTTGGCATTGTTCA35480.10877459653300901No Hit
TACGAACTTCCACCGGATATAGAATAGGTGTCGAAGACGATGAGGACTTA35080.1075482763917124No Hit
TGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAGGAGTTT34340.10527958413031367No Hit
TGAGAAAAGAAATTATACATTTCAGAAACGTCAATAGGGTATCATCATCG33900.10393063197488742No Hit
TGGAAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCC33490.10267365383005839No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAGTC56450.099.7056052
TACTTGT97450.092.9811
TGACGAA56900.087.6820451
TATGATG83900.084.973571
TTCAGTC42700.073.391741
TCGGGCC5300.069.486191
TAGTGAG43200.068.8428041
TGGACGA22700.068.567871
TTGATGT92650.068.5487061
TCTAAGC13600.068.464331
TTCCACC74300.066.963179
TGTGAGG25600.065.9575961
GACGAAC75300.065.702742
ACGAACT77100.063.9866183
CTTCCAC77650.063.9847378
TCCTAAG20300.062.9825551
TGTCAGT93400.062.120951
AACTTCC80650.061.775076
AGTCAGG91650.061.184245
GAACTTC81400.061.0351645
Produced by FastQC (version 0.11.2)