FastQCFastQC Report
Sun 15 Jan 2017
HH75LAFXX_n01_tpq1ss_1_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH75LAFXX_n01_tpq1ss_1_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2916288
Sequences flagged as poor quality0
Sequence length145
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTTGATGGAAGCAAGTACTTTTTAGATACCCAGTAAAAGTCTCCATAT132680.45496192419953035No Hit
TATGAACAACAATTGTTGTAGTCGCAACTACGGTAATTGGTCCTAAAAAA99900.34255875962867866No Hit
TGTGATAACAACCTTCTTGGCACCAGCGTCAATGTGCTTTTGAGCAGTGT78400.2688349024513354No Hit
TCATTATCCTCATCAAGATTGCTTTATTTAAGCCTTGGCAACATATTCGA75950.2604338117497312No Hit
TCTTGCTGTCTCTAGCAGCTTCTAAAGCAGCGACGGCAGTAGAAGAAGAG67790.2324530361884697No Hit
TATTGGGTGCAAAGCCTTGTAGACGTTGTAAACTTGTTCTGGCTTGGTGT66200.22700089977395924No Hit
TATAATGGTTCACCGTGTTCCTTGAAGTAAGCTTCATCAGCTTCCAACAT62650.21482789079816533No Hit
TAGCAGCTGGAGCACCGGTAATGATAACCTTCTTGTTTTCAGAAATTAAT60250.20659825092720607No Hit
TGAAGAAATTAAAAGAATTTCTACTAAATCCCAATTGTTATATATTTGTT60170.2063239295981741No Hit
TGTAGCAATGTCAGTGATCATAGCAGTGGTTTCAGAAATGTTGGCAGACA56850.19493959444334716No Hit
TCCACCGTTCAAAACGTTCAAGAATGGAACTGGCAAAACGTATGGAGAGG54640.18736146772883885No Hit
TTCATTATCCTCATCAAGATTGCTTTATTTAAGCCTTGGCAACATATTCG51890.1779316720433647No Hit
TATCATTATCCTCATCAAGATTGCTTTATTTAAGCCTTGGCAACATATTC47940.16438705642241094No Hit
TTAATGGTTCACCGTGTTCCTTGAAGTAAGCTTCATCAGCTTCCAACATA46210.1584548576820945No Hit
TATGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTG44560.15279698027081No Hit
TCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCACATTCT44150.15139108345952113No Hit
TCTCTGGAACAGCTGATGAAGCAGGTGTTGTTGTCTGTTGAGAGTTAGCC40430.1386351416595343No Hit
TTGAGAAAAGAAATTATACATTTCAGAAACGTCAATAGGGTATCATCATC39230.13452032172405468No Hit
TAGTGATAACAACCTTCTTGGCACCAGCGTCAATGTGCTTTTGAGCAGTG38570.13225717075954088No Hit
TCCTAAGCGCAGCATATCATGAAAGAGCGGTGAGACATGCTTGGAAGGGG37570.12882815414664123No Hit
TAATAATGGTTCACCGTGTTCCTTGAAGTAAGCTTCATCAGCTTCCAACA37300.12790231966115828No Hit
TCAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTTGGCGT36340.1246104637127746No Hit
TGGTAGCAATGTCAGTGATCATAGCAGTGGTTTCAGAAATGTTGGCAGAC36310.12450759321438759No Hit
TATTTGCGTGTACTTTCACTTGTATGGACATTATTGATGGTGGGTACGGA36220.12419898171922664No Hit
TGTTATTAGATGTGGATACATTGTGAGCCCTGGCTGTTTTCGATTTCGAA33990.11655227467246033No Hit
TAGAATAATGGTTCACCGTGTTCCTTGAAGTAAGCTTCATCAGCTTCCAA33790.11586647134988039No Hit
TTGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAA32590.11175165141440078No Hit
TGAGAATAATGGTTCACCGTGTTCCTTGAAGTAAGCTTCATCAGCTTCCA31700.10869982662892005No Hit
TTGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTGG31340.10746538064827617No Hit
TTAGCAATGTCAGTGATCATAGCAGTGGTTTCAGAAATGTTGGCAGACAT31190.10695102815634122No Hit
TTGAAATTGTAGGTCACCAATAGCGTTAATTGGTATATTTCTTTTTTGAG30790.10557942151118134No Hit
TGTGCTAGATCTCTTCTTTGATGGAAGAATATTAGATTCAGATATGTTGG30230.1036591722079575No Hit
TCTAAGCGCAGCATATCATGAAAGAGCGGTGAGACATGCTTGGAAGGGGA30020.10293907871924857No Hit
TAAAAGAAATCATCAAATCATTCATTCTTCAGACTTATTTCTTACCTTGG29230.10023015559505782No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGCC2300.072.507951
TCCCGCA7900.069.486791
TGCGGCT10650.066.550731
TCGGGTC5350.066.2397461
TCCACCG13500.065.883771
TCGGCGC2900.062.29851
TCCTAAG9400.062.0945781
TCGCGGC3600.057.9056551
TGCCCGT7500.057.4424171
TCGGGCT5300.056.3760761
TATTGGG17800.054.2621541
TCGGGAC3800.051.2007941
TCGGCCG2450.051.0515171
TCCGGCC3500.049.633421
TGGGCCC11850.048.0836831
CGGCTCA4400.045.797333
TGCCCCT8050.045.749071
TGTCCCG4300.045.2472151
TCTAAGC10650.045.019611
CTCCCCC5300.044.576432
Produced by FastQC (version 0.11.2)