FastQCFastQC Report
Sun 15 Jan 2017
HH75LAFXX_n01_tpq1ss_19_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH75LAFXX_n01_tpq1ss_19_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2866274
Sequences flagged as poor quality0
Sequence length145
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTG359621.2546602313665756No Hit
TTGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTGG354991.2385068559391044No Hit
TGACGAACTTCCACCGGATATAGAATAGGTGTCGAAGACGATGAGGACTT317111.106349218532492No Hit
TGTCAGTCAGGACCATAGCTACTGGCAATTATCGAGATGCTGCTGAAAGA259790.9063683374304061No Hit
TTCAGTCAGGACCATAGCTACTGGCAATTATCGAGATGCTGCTGAAAGAT184850.6449139196043365No Hit
TAGTGAGGGCACTATATGGGTGTGATTTTTCATCACATACAATGGCTGAT176980.6174566702276196No Hit
TCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCACATTCT140320.4895554298018961No Hit
TGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTGGG124690.4350247045467391No Hit
TGTGCTAGATCTCTTCTTTGATGGAAGAATATTAGATTCAGATATGTTGG119070.41541736763477605No Hit
TATGAAATTAGCTGGCGTATTTGATCTGGATGACGTTCAAGACTCGGTAC108380.3781215613022342No Hit
TAGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAAGA101930.355618478903273No Hit
TGGACGAACTTCCACCGGATATAGAATAGGTGTCGAAGACGATGAGGACT97800.3412095284679692No Hit
TCGATGGTCTTGGGGCTCAGTACTTAAGTAGGTCTTGTGCTACTCTTGTA90040.31413605258952915No Hit
TCAGTCAGGACCATAGCTACTGGCAATTATCGAGATGCTGCTGAAAGATT89560.3124614045970483No Hit
TGCCTTCCATTGCCTCCTTTTTTTCTCTTCCAGAACTCTCTCAGCAGTAT86570.3020297431438865No Hit
TCATCGATTGAAGGTGAACGGTGTATAATCCTTTTCTCAGTCTCTTGGTC84340.29424960767881925No Hit
TGTGAGGGCACTATATGGGTGTGATTTTTCATCACATACAATGGCTGATT83920.2927842906853986No Hit
TAAAGTGAGGGCACTATATGGGTGTGATTTTTCATCACATACAATGGCTG76490.266862135301789No Hit
TCTTATAAGGGTTCGTGATGCTCCTGAATCGAGAAGGAGGTGTCCAGGGA75120.26208241082324996No Hit
TCAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTTGGCGT73560.2566398048476873No Hit
TTGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTGGGCC72970.2545813833569296No Hit
TATGATATTTGTGAAGGCGGTACGCATTGGTCAACTCAATGCATATTATG70310.24530104239859832No Hit
TGTAGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAA65400.22817078897551316No Hit
TTGAGAAAAGAAATTATACATTTCAGAAACGTCAATAGGGTATCATCATC64550.22520526648882833No Hit
TGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAG64490.22499593548976826No Hit
TTAGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAAG62550.21822756652015823No Hit
TTGCTAGATCTCTTCTTTGATGGAAGAATATTAGATTCAGATATGTTGGG52660.18372284017508445No Hit
TTGCTACTTTCACGCACAGTAGGGTTGAGTCTGATGCACCACTGACATTG52470.1830599586780608No Hit
TGTTATTAGATGTGGATACATTGTGAGCCCTGGCTGTTTTCGATTTCGAA49450.17252363172536891No Hit
TGGTCAGTCAGGACCATAGCTACTGGCAATTATCGAGATGCTGCTGAAAG49210.1716863077291285No Hit
TTATGAAATTAGCTGGCGTATTTGATCTGGATGACGTTCAAGACTCGGTA48920.17067454123367132No Hit
TAGCATCGATTGAAGGTGAACGGTGTATAATCCTTTTCTCAGTCTCTTGG48250.16833701174416682No Hit
TCTTTAAATGGGTCAGGGAATTCGGTAGGAGATTCTGGAGGTAGATCAGG48110.16784857274635992No Hit
TTGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAA48060.16767413024714317No Hit
TCCTAAGCGCAGCATATCATGAAAGAGCGGTGAGACATGCTTGGAAGGGG48010.1674996877479264No Hit
TATATGAAATTAGCTGGCGTATTTGATCTGGATGACGTTCAAGACTCGGT47660.16627859025340913No Hit
TGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT46390.1618477507733036No Hit
TTGATATTTGTGAAGGCGGTACGCATTGGTCAACTCAATGCATATTATGT46080.16076620727815974No Hit
TGCGGAAACTGTGATTGTGGCCCAGGTGGAAAGTACATAGGCGACATTTG45740.15957999828348582No Hit
TCTCGTTAATCCATTCATGCGCGTCACTAATTAGATGACGAGGCATTTGG45480.15867289728755868No Hit
TGAGCAGTTGTAATGCCAGGTGGATCGGTCCACAGTCAACATCCAGTCGA45110.15738202279335473No Hit
TCACGGTATGAGCGGAGGCGATGGTCTTGGGGCTCAGTACTTAAGTAGGT44700.15595159429977734No Hit
TACAATGTACAAAGCCTATAAGGGTTTGGCAGTCTCATACTATGCTAACC43830.15291629481340585No Hit
TCTCTGGAACAGCTGATGAAGCAGGTGTTGTTGTCTGTTGAGAGTTAGCC42660.14883434033173382No Hit
TAGCAGTTGTAATGCCAGGTGGATCGGTCCACAGTCAACATCCAGTCGAA42450.14810168183502345No Hit
TGCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCT42010.14656658784191604No Hit
TATTTCTTTTTTGAGCATCAACTACGTTTATGTCAGGATTAGATGATGCT41560.14499660534896525No Hit
TCTGTGATTGTGGCCCAGGTGGAAAGTACATAGGCGACATTTGATAAGGT41440.14457794335084503No Hit
TCTAAGCGCAGCATATCATGAAAGAGCGGTGAGACATGCTTGGAAGGGGA40160.14011221537089613No Hit
TGATGATATTTGTGAAGGCGGTACGCATTGGTCAACTCAATGCATATTAT40080.13983310737214932No Hit
TAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTTGGCGTT39080.13634425738781428No Hit
TAGATGATATTTGTGAAGGCGGTACGCATTGGTCAACTCAATGCATATTA38860.13557671039126057No Hit
TAAGTGAGGGCACTATATGGGTGTGATTTTTCATCACATACAATGGCTGA38120.1329949614028526No Hit
TGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC37730.13163430990896197No Hit
TCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCTC37500.13083187441256489No Hit
TGCTAGATCTCTTCTTTGATGGAAGAATATTAGATTCAGATATGTTGGGG34970.12200508395219718No Hit
TGTTCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCACAT34880.12169108745360702No Hit
TCTACGAAGTACTCTGATAACTAACTTTGCAATGTTCAGGTGCGAGGATG34800.12141197945486021No Hit
TACGAACTTCCACCGGATATAGAATAGGTGTCGAAGACGATGAGGACTTA34800.12141197945486021No Hit
TAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAGGAG33750.1177486869713084No Hit
TGCATGATGGGGGTTCTCTGGAACAGCTGATGAAGCAGGTGTTGTTGTCT33530.11698113997475469No Hit
TTGCGGAAACTGTGATTGTGGCCCAGGTGGAAAGTACATAGGCGACATTT31730.11070121000295156No Hit
TCCGGTTCGCAATACTACAAGCTTTGATAAGCACGTGTGTTGCTTCTAAA30990.10811946101454363No Hit
TAGCAGATCTTATAAGGGTTCGTGATGCTCCTGAATCGAGAAGGAGGTGT30570.10665414402112289No Hit
TAAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCCCT29890.10428172603177506No Hit
TATTTGATGGAAGCAAGTACTTTTTAGATACCCAGTAAAAGTCTCCATAT29720.10368862153443809No Hit
TGAACACGGTATGAGCGGAGGCGATGGTCTTGGGGCTCAGTACTTAAGTA29040.10131620354509024No Hit
TATTAGATGTGGATACATTGTGAGCCCTGGCTGTTTTCGATTTCGAATTA28830.10058354504837988No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAGTC38800.0103.696262
TGACGAA44200.0102.975821
TATGATG61900.081.725391
GACGAAC55750.080.270742
TCCTAAG8050.078.552811
ACGAACT57650.077.504683
TGCGCCT12050.076.120761
TGCCTGC12700.074.960611
TGGACGA18150.074.274771
TAGTGAG34400.073.1250841
TCGGGAC3400.069.489031
AGTCCAA34850.068.990546
TCGCGCG3550.068.510311
CCAGTCC35000.068.496324
TCGGGCC3250.068.419971
CTTCCAC65500.068.320858
CGAACTT67250.066.337484
GAACTTC68150.065.460285
TTCCACC69250.064.622299
TGTGAGG18600.063.1378751
Produced by FastQC (version 0.11.2)