FastQCFastQC Report
Sun 15 Jan 2017
HH75LAFXX_n01_tpq1ss_18_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH75LAFXX_n01_tpq1ss_18_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3101321
Sequences flagged as poor quality0
Sequence length145
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTG324991.0479082945622205No Hit
TGTCAGTCAGGACCATAGCTACTGGCAATTATCGAGATGCTGCTGAAAGA298950.9639440741542071No Hit
TTGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTGG270930.8735954775400546No Hit
TGACGAACTTCCACCGGATATAGAATAGGTGTCGAAGACGATGAGGACTT223400.7203382042684392No Hit
TAGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAAGA159910.5156189894564284No Hit
TAGTGAGGGCACTATATGGGTGTGATTTTTCATCACATACAATGGCTGAT150750.48608318842196596No Hit
TTCAGTCAGGACCATAGCTACTGGCAATTATCGAGATGCTGCTGAAAGAT147040.47412054411652327No Hit
TGTAGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAA129150.41643544799135596No Hit
TGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTGGG127600.4114375777289742No Hit
TATGAAATTAGCTGGCGTATTTGATCTGGATGACGTTCAAGACTCGGTAC110980.3578475107865326No Hit
TCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAACTACAAATGC107910.34794850323458937No Hit
TCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCACATTCT97180.31335034328919836No Hit
TGTGAGGGCACTATATGGGTGTGATTTTTCATCACATACAATGGCTGATT93970.30299991519742714No Hit
TATGAACAACAATTGTTGTAGTCGCAACTACGGTAATTGGTCCTAAAAAA93550.30164565357794304No Hit
TGGACGAACTTCCACCGGATATAGAATAGGTGTCGAAGACGATGAGGACT89310.2879740600860085No Hit
TATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAACTACAAAT86770.2797840017205571No Hit
TTAGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAAG84890.2737220687571522No Hit
TGCCTTCCATTGCCTCCTTTTTTTCTCTTCCAGAACTCTCTCAGCAGTAT81150.26166269147888915No Hit
TATGATATTTGTGAAGGCGGTACGCATTGGTCAACTCAATGCATATTATG77950.2513445077113914No Hit
TCGATGGTCTTGGGGCTCAGTACTTAAGTAGGTCTTGTGCTACTCTTGTA76980.24821680825686862No Hit
TTGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAA76410.24637888177328304No Hit
TCAGTCAGGACCATAGCTACTGGCAATTATCGAGATGCTGCTGAAAGATT74280.23951084070304238No Hit
TTGAGAAAAGAAATTATACATTTCAGAAACGTCAATAGGGTATCATCATC73900.238285556380652No Hit
TCAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTTGGCGT69780.2250008947799986No Hit
TATTTGATGGAAGCAAGTACTTTTTAGATACCCAGTAAAAGTCTCCATAT69240.22325970126923336No Hit
TCTTATAAGGGTTCGTGATGCTCCTGAATCGAGAAGGAGGTGTCCAGGGA66550.21458597803968052No Hit
TGTGCTAGATCTCTTCTTTGATGGAAGAATATTAGATTCAGATATGTTGG64080.20662162994414318No Hit
TGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAAGAA62750.2023331348157769No Hit
TATATGAAATTAGCTGGCGTATTTGATCTGGATGACGTTCAAGACTCGGT57420.1851469099780384No Hit
TCCTAAGCGCAGCATATCATGAAAGAGCGGTGAGACATGCTTGGAAGGGG56330.18163227863223447No Hit
TTGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTGGGCC55240.1781176472864305No Hit
TGGTCAGTCAGGACCATAGCTACTGGCAATTATCGAGATGCTGCTGAAAG55180.17792418134078994No Hit
TCTAAGCGCAGCATATCATGAAAGAGCGGTGAGACATGCTTGGAAGGGGA54560.17592503323583725No Hit
TCATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAACTACAAA53180.17147531648610384No Hit
TAAAGTGAGGGCACTATATGGGTGTGATTTTTCATCACATACAATGGCTG51570.1662839802780815No Hit
TCTACGAAGTACTCTGATAACTAACTTTGCAATGTTCAGGTGCGAGGATG49790.16054449055741088No Hit
TGATGATATTTGTGAAGGCGGTACGCATTGGTCAACTCAATGCATATTAT49360.15915798461365335No Hit
TAGCAGCTGGAGCACCGGTAATGATAACCTTCTTGTTTTCAGAAATTAAT48740.15715883650870066No Hit
TTGATATTTGTGAAGGCGGTACGCATTGGTCAACTCAATGCATATTATGT48320.15580457488921656No Hit
TTATGAAATTAGCTGGCGTATTTGATCTGGATGACGTTCAAGACTCGGTA48280.15567559759212282No Hit
TGTTATTAGATGTGGATACATTGTGAGCCCTGGCTGTTTTCGATTTCGAA47280.1524511651647798No Hit
TCTCCCGTAACACTATCTCTGATACCCTACCCTTTTCTTGCAAACCGCCA45420.1464537208499217No Hit
TGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAG45350.14622801058000767No Hit
TTGCTACTTTCACGCACAGTAGGGTTGAGTCTGATGCACCACTGACATTG45350.14622801058000767No Hit
TGTTGACATTGAGAATATAGATAGTTATAAGAAGACAAGGATGATTGCCA45090.14538965814889848No Hit
TGGGCACTATATGGGTGTGATTTTTCATCACATACAATGGCTGATTTTGG41320.13323354789781516No Hit
TACAATGTACAAAGCCTATAAGGGTTTGGCAGTCTCATACTATGCTAACC40730.13133113276568276No Hit
TAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAGGAG40210.12965442790346438No Hit
TCACGGTATGAGCGGAGGCGATGGTCTTGGGGCTCAGTACTTAAGTAGGT39600.1276875241227851No Hit
TAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTTGGCGTT39590.12765527979851166No Hit
TGCCGGCTGTGCTCGCGCATTTATCCGGTGCATAGCGTCCATTATTCTTA39180.126333262503301No Hit
TGCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCT38890.1253981770993715No Hit
TAGCAGTTGTAATGCCAGGTGGATCGGTCCACAGTCAACATCCAGTCGAA37620.12130314791664586No Hit
TGAACACGGTATGAGCGGAGGCGATGGTCTTGGGGCTCAGTACTTAAGTA36350.11720811873392016No Hit
TGAGCAGTTGTAATGCCAGGTGGATCGGTCCACAGTCAACATCCAGTCGA34590.11153311766179637No Hit
TCATCGATTGAAGGTGAACGGTGTATAATCCTTTTCTCAGTCTCTTGGTC34450.11108169712196833No Hit
TATGAGAAAAGAAATTATACATTTCAGAAACGTCAATAGGGTATCATCAT34450.11108169712196833No Hit
TGTTGGCGATTATACTGCATTCCTGAAAACTAACTTTTCCGAGATAGCTG32230.10392345713326676No Hit
TCTCTGGAACAGCTGATGAAGCAGGTGTTGTTGTCTGTTGAGAGTTAGCC31820.1026014398380561No Hit
TTGCTAGATCTCTTCTTTGATGGAAGAATATTAGATTCAGATATGTTGGG31550.10173084308267347No Hit
TCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCTC31510.10160186578557977No Hit
TGGGCATTTTGTTAACAGTTATGAACTTGTTCCTAGTATACTGACCAGGA31470.10147288848848604No Hit
TGATATTTGTGAAGGCGGTACGCATTGGTCAACTCAATGCATATTATGTA31020.10002189389618164No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAGTC45900.099.774052
TGACGAA41500.084.397061
TCGGGCC3850.083.031331
ACGAACT49350.069.000743
GACGAAC49700.068.5148242
TATGATG74750.068.424481
TCTAAGC12150.068.06371
TCCTAAG12650.066.4721451
TGTAGGA28700.065.1351241
TATCGCG21300.064.925961
TTAGGAG21200.063.5932041
AGTCAGG71700.063.581325
TGCCGGC18500.063.483351
TCGGGTC7350.063.347921
TGGGCCC10850.062.7684141
TGGACGA19550.060.4292261
TGCGGCT10950.060.2912561
TAGTGAG36350.060.0302431
CAGTCCA25250.059.4479985
TGTGAGG23300.059.0546531
Produced by FastQC (version 0.11.2)