FastQCFastQC Report
Sun 15 Jan 2017
HH75LAFXX_n01_tpq1ss_17_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH75LAFXX_n01_tpq1ss_17_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4320611
Sequences flagged as poor quality0
Sequence length145
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAG558941.2936596236041615No Hit
TGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC454971.053022361883539No Hit
TGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT453141.0487868498228607No Hit
TCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAG406420.9406539954649932No Hit
TATGAACAACAATTGTTGTAGTCGCAACTACGGTAATTGGTCCTAAAAAA232970.5392061446864806No Hit
TCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTC227680.5269625059974157No Hit
TGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAAGT225160.5211299975859895No Hit
TGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT222990.5161075597872615No Hit
TATGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTG218010.5045814122123006No Hit
TTGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTGG197150.4563012036954958No Hit
TCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGT187320.43354979191600446No Hit
TGCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATT154670.3579817761885993No Hit
TGCCTTCCATTGCCTCCTTTTTTTCTCTTCCAGAACTCTCTCAGCAGTAT139500.32287100134680025No Hit
TGTCAGTCAGGACCATAGCTACTGGCAATTATCGAGATGCTGCTGAAAGA138460.3204639343833546No Hit
TCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC133280.3084748893154232No Hit
TTGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAA122470.28345527982037727No Hit
TCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCACATTCT120140.278062524027273No Hit
TAGCAGCTGGAGCACCGGTAATGATAACCTTCTTGTTTTCAGAAATTAAT108940.2521402644209349No Hit
TCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGT103430.2393874384896025No Hit
TCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTC89730.2076789602211354No Hit
TCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG80660.18668655891493124No Hit
TGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTGGG74820.1731699521201978No Hit
TTGATCTTGGAGATCGCGTCTGGGTTGAATTGTGCAGTGGACACGAGAGG74700.17289221362441562No Hit
TTGAGAAAAGAAATTATACATTTCAGAAACGTCAATAGGGTATCATCATC73830.1708786095299947No Hit
TTGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAA72650.16814751432146982No Hit
TGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAG70100.16224557128609818No Hit
TATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAAGTTAT70000.16201412253961303No Hit
TCGATTATACTGCATTCCTGAAAACTAACTTTTCCGAGATAGCTGATGCT69590.16106518267902387No Hit
TAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAGGAG69060.1598385043226525No Hit
TCTCTCTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTACTTGTTCGCT66630.15421429978306309No Hit
TTCAGTCAGGACCATAGCTACTGGCAATTATCGAGATGCTGCTGAAAGAT65970.152686738056261No Hit
TCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTT63950.14801147337726075No Hit
TCTAAGCGCAGCATATCATGAAAGAGCGGTGAGACATGCTTGGAAGGGGA61360.14201695084329508No Hit
TCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAACTACAAATGC61000.1411837353559485No Hit
TATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAACTACAAAT60300.13956359413055236No Hit
TCCTAAGCGCAGCATATCATGAAAGAGCGGTGAGACATGCTTGGAAGGGG59890.1386146542699632No Hit
TCTCCCGTAACACTATCTCTGATACCCTACCCTTTTCTTGCAAACCGCCA59370.13741112078824036No Hit
TGTTGGCGATTATACTGCATTCCTGAAAACTAACTTTTCCGAGATAGCTG56230.13014363014860628No Hit
TGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACCA53370.12352419599913067No Hit
TCATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAACTACAAA53200.12313073313010592No Hit
TAGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAAGA52210.1208393905399028No Hit
TGTAGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAA51990.12033020329763545No Hit
TATGAAGCCTTGTTGGCAATGATGCTAGACAGGTTTAACAACATAGAGTC50230.11625670535949661No Hit
TAAGCAGCTGGAGCACCGGTAATGATAACCTTCTTGTTTTCAGAAATTAA47830.11070193544385272No Hit
TGCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCT47680.11035476232412499No Hit
TATACGGGCCAACCCCCATGGGGCGCTATTTTGAGTGTCAAATTCTGTAG47240.1093363878395903No Hit
TCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAGTTCTTTT46880.1085031723522437No Hit
TATGAGAAAAGAAATTATACATTTCAGAAACGTCAATAGGGTATCATCAT44600.10322614093238201No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCCT63000.0109.525641
TGCCTGC74250.0105.097021
TCCCGTT38350.095.6699751
TCGCCTG29250.085.998291
TCGCGCG4350.084.663021
TCGGGCC5100.079.025081
TCGCGGC8950.076.863351
GCCCTTC110550.075.2389142
GTCAGTC26500.074.994132
CCCTTCC116000.070.085793
TGCCCTT131750.069.513951
TGCCCGT16900.067.4317251
TGGCCCC15000.066.708071
TGCGCGC49750.066.624271
CCTTCCC123000.065.703244
TGGCCCG14000.065.516851
TCCCCCG6800.064.378191
TGCCCGC17300.063.0609741
GCCCTCT40050.062.1137352
TGCCCCG7150.060.254961
Produced by FastQC (version 0.11.2)