FastQCFastQC Report
Sun 15 Jan 2017
HH75LAFXX_n01_tpq1ss_16_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH75LAFXX_n01_tpq1ss_16_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3626438
Sequences flagged as poor quality0
Sequence length145
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGAACAACAATTGTTGTAGTCGCAACTACGGTAATTGGTCCTAAAAAA264430.729172813653508No Hit
TATTTGATGGAAGCAAGTACTTTTTAGATACCCAGTAAAAGTCTCCATAT182420.5030280401870927No Hit
TCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCACATTCT144230.39771809141642567No Hit
TCAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTTGGCGT130510.3598848236203128No Hit
TGTGCTAGATCTCTTCTTTGATGGAAGAATATTAGATTCAGATATGTTGG99410.2741257399133806No Hit
TTGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAA89590.24704682666572544No Hit
TGTTATTAGATGTGGATACATTGTGAGCCCTGGCTGTTTTCGATTTCGAA79150.21825824679754624No Hit
TCTCTGGAACAGCTGATGAAGCAGGTGTTGTTGTCTGTTGAGAGTTAGCC71990.19851435485730073No Hit
TGCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCT63200.17427569422116138No Hit
TCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCTC62420.17212482331146983No Hit
TGCCTTCCATTGCCTCCTTTTTTTCTCTTCCAGAACTCTCTCAGCAGTAT61770.17033243088672687No Hit
TAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTTGGCGTT58130.1602950333081663No Hit
TCTTTAAATGGGTCAGGGAATTCGGTAGGAGATTCTGGAGGTAGATCAGG57950.15979867848285287No Hit
TCAAATATTTGAAAAGTGTTATACAAGAAGGTGAGTTCATCATCAGTGAT54740.15094701743142994No Hit
TTGCTAGATCTCTTCTTTGATGGAAGAATATTAGATTCAGATATGTTGGG54540.1503955120699706No Hit
TCCCTGTTGTTCTTCATAAATAGCATGGATATCTAAGAACAGTTCAGCGA53360.1471416304373603No Hit
TATTAGATGTGGATACATTGTGAGCCCTGGCTGTTTTCGATTTCGAATTA52350.14435652836199048No Hit
TATGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTG51030.1407165929763586No Hit
TGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAG50710.13983418439802361No Hit
TCTTATGTATAATTCTTCTTTGATGTCTGCATACAAATATGCCGAAGATA49280.13589092106358913No Hit
TTTCATCATCAGTGATCTGACGTACGGGTTTTCCGTTTACTGTCGGAATA42710.11777396993964877No Hit
TAAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCCCT42310.11667095921673001No Hit
TAGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAAGA42140.11620217965948956No Hit
TTGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTGG42010.11584370117454097No Hit
TAGTATATGTTGAGCAAGTGTGTTGATGTAGTATAGTAAGTCAAATCTAA41020.11311374963531708No Hit
TGAAGAAATTAAAAGAATTTCTACTAAATCCCAATTGTTATATATTTGTT40860.11267254534614958No Hit
TCTTATAAGGGTTCGTGATGCTCCTGAATCGAGAAGGAGGTGTCCAGGGA40760.1123967926654199No Hit
TTATTAGATGTGGATACATTGTGAGCCCTGGCTGTTTTCGATTTCGAATT40360.11129378194250115No Hit
TATCAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTTGGC39820.10980471746656086No Hit
TAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAGGAG39060.10770899709301524No Hit
TGCCTCGATATATTTTTCTTTTTCTTTAATATCTTTATTATAGGTGATTG38880.1072126422677018No Hit
TCATCGATTGAAGGTGAACGGTGTATAATCCTTTTCTCAGTCTCTTGGTC37760.10412421224352932No Hit
TCGGGTCAAGATCATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAA37570.10360028215014293No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGCC4300.082.417231
TCGGGCT7700.073.0988541
TGCCCGT12500.072.268591
TCCCGCA14250.068.757571
TCGGGAC5000.066.709471
CAGTCCA39900.063.2193455
TCCAAGC44800.062.5091068
TATGAAC62150.062.0537571
TGGGCCC14800.061.9767041
CCAGTCC40950.061.5983354
GTCCAAG45850.060.7736477
TAGCGAT17450.058.9362531
TCCCGGA8950.058.2310331
TCGCGGC4700.056.182621
TCGGGTC19300.056.16731
TGGCCCG11700.055.8287961
TCGGCCG6300.054.0470281
AGTCCAA47950.052.316046
TGCGGCT15450.051.723231
CCCCCCG34600.050.828514116-117
Produced by FastQC (version 0.11.2)