FastQCFastQC Report
Sun 15 Jan 2017
HH75LAFXX_n01_tpq1ss_15_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH75LAFXX_n01_tpq1ss_15_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3888549
Sequences flagged as poor quality0
Sequence length145
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTCGCTG3341008.591893788659986No Hit
TATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTCG1830504.707411427758786No Hit
TGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTC1375033.536100483753709No Hit
TCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTT1107842.8489804294609637No Hit
TATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAA1036892.666521625418633No Hit
TGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGC841332.163609099435291No Hit
TTTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTCGC777151.998560388463666No Hit
TCGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTT704741.8123469705537978No Hit
TTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTCGCT697441.7935739012161092No Hit
TGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTA687881.768988895343739No Hit
TGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATC480091.234625049086433No Hit
TGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG417951.0748225109160257No Hit
TGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACG322130.828406688458857No Hit
TGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTCGCTGC308730.7939465337841956No Hit
TCGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAG295120.758946331909409No Hit
TCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTC291000.7483511201736175No Hit
TCACGGAATTCTGCAATTCACATTACGTATCGCATTTCGCTGCGTTCTTC227790.5857969129359049No Hit
TGGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTC224530.5774133230672932No Hit
TCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTAT222830.5730415123996123No Hit
TCCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTCGCT215800.5549627894620847No Hit
TGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGTTC213770.5497423331942068No Hit
TACGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTCGCT212090.5454219555932046No Hit
TCGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGT170720.43903265716852224No Hit
TAGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGT149020.38322777982224215No Hit
TGTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTCGC143230.3683379070187877No Hit
TCCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAA132670.3411812478124874No Hit
TGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT132200.3399725707455403No Hit
TCTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTCGC128340.33004598887657066No Hit
TGGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAA126320.3248512491420321No Hit
TCTGCAATTCACATTACGTATCGCATTTCGCTGCGTTCTTCATCGATGCG126150.3244140680752641No Hit
TGTCGTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCC125910.32379687127512086No Hit
TGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC125380.322433895008138No Hit
TTGACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGC121460.31235301393913256No Hit
TCCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAG119090.3062581955377186No Hit
TTGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTCGCTGCGT105660.2717208912630392No Hit
TACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGC105590.27154087552966416No Hit
TGCCCTTCCCTTTCAACAATTTCACGTACTTTTTCACTCTCTTTTCAAAG104240.2680691435288587No Hit
TGCCGCTTCACTCGCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCT101260.26040561659374745No Hit
TCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTC98500.25330785339210077No Hit
TACGGAATTCTGCAATTCACATTACGTATCGCATTTCGCTGCGTTCTTCA96130.24721303499068678No Hit
TGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACGGTCTA90590.2329660755207148No Hit
TCCCGGTTGGTTTCTTTTCCTCCGCTTATTGATATGCTTAAGTTCAGCGG87760.225688296585693No Hit
TCAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAGCA85510.21990207658435063No Hit
TGCTACTACCACCAAGATCTGCACTAGAGGCCGTTCGACCCGACCTTACG85370.21954204511760042No Hit
TCCCTTGTCCGTACCAGTTCTAAGTTGATCGTTAATTGTAGCAAGCGACG81810.21038695924880976No Hit
TGCGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCA81100.20856108538171952No Hit
TGCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTCGCT79390.20416355818069928No Hit
TCGTTAATTGTAGCAAGCGACGGTCTACAAGAGACCTACCAAGGCCGTCT75650.19454557471180126No Hit
TATCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTCGC71270.18328173310918802No Hit
TACGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGT69210.17798412724129234No Hit
TCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC68850.17705833204107754No Hit
TGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCT68830.17700689897439892No Hit
TCCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGT66520.17106637977302072No Hit
TCCCGGATCAGCCCCGAATGGGACCTTGAATGCTAGAACGTGGAAAATGA66420.17080921443962774No Hit
TCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGATCGATAAGATCG65120.16746606510551879No Hit
TTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGT64120.16489441177158884No Hit
TTGCCGCTTCACTCGCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCC61300.15764234936990637No Hit
TGCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCTCCGCTTATTGAT59150.15211329470195695No Hit
TGCATGTATTAGCTCTAGAATTACCACAGTTATACCATGTAGTAAAGGAA58080.1493616256346519No Hit
TGGCGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCG57870.1488215784345266No Hit
TTACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCAAG57610.1481529485677048No Hit
TTATACAAATGATTTATCCCCACGCAAAATGACATTGCAATTCGCCAGCA57180.14704713763411492No Hit
TGTTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTCG57110.14686712190073983No Hit
TCCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCC55080.14164666563286202No Hit
TCACGCAAAATGACATTGCAATTCGCCAGCAAGCACCCAAGGCCTTTCCG54180.13933217763232505No Hit
TCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG53530.13766060296527058No Hit
TGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTTCGCTGCGTT52840.1358861621648589No Hit
TGCGCTCAAACAGGCATGCCCCCTGGAATACCAAGGGGCGCAATGTGCGT51100.1314114853638208No Hit
TCCGGCACCTTAACTCTACGTTCGGTTCATCCCGCATCGCCAGTTCTGCT50670.13030567443023092No Hit
TGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAGTAGATAGGGACAGTGG49800.12806833602971185No Hit
TCCCTTATCTACATTATTCTATCAACTAGAGGCTGTTCACCTTGGAGACC49680.12775973762964027No Hit
TGCCCTCTCCAAATTACAACTCGGGCACCGAAGGTACCAGATTTCAAATT49050.1261395960292644No Hit
TGCAATGTGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACA47950.12331077736194145No Hit
TCCCGTTGTACATCTAAGTCGTATACAAATGATTTATCCCCACGCAAAAT47200.12138203736149396No Hit
TATGCGTTCAAAGATTCGATGATTCACGGAATTCTGCAATTCACATTACG47170.12130488776147606No Hit
TCGGACGGGAAACGGTGCTTTCTGGTAGATATGGCATCGATAAGATCGGA46540.1196847461611002No Hit
TCTGCGTTCTTCATCGATGCGAGAACCAAGAGATCCGTATCGATAAGATC44500.1144385733598831No Hit
TCGCCGTTACTAAGGCAATCCCGGTTGGTTTCTTTTCCTCCGCTTATTGA42500.10929526669202316No Hit
TAGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTT42350.10890951869193367No Hit
TGCCGACTTCCCTTATCTACATTATTCTATCAACTAGAGGCTGTTCACCT42190.10849805415850489No Hit
TCGGAATTCTGCAATTCACATTACGTATCGCATTTCGCTGCGTTCTTCAT42150.10839518802514768No Hit
TGGATTCGATGATTCACGGAATTCTGCAATTCACATTACGTATCGCATTT39720.10214607042369789No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTATAC90600.0138.057821
TCGCTCA74800.0136.65571
TCCCACG33800.0136.305541
TACGGGC6150.0133.328671
TGATTCG151300.0132.731981
TCCGATG24150.0130.921481
TAGGCGC1700.0130.8031
TGTTCAA55000.0130.765841
TAGGGCT3000.0129.712981
TGCCTGT2500.0127.859931
TCGCGCG5400.0127.3966751
TGTCCCC9600.0127.3966751
TCGGGCG4800.0125.948991
TGGCGCA26850.0124.226311
TACGATG24800.0123.56731
TGAGGCG5850.0123.536161
TCGTTCA27950.0123.066551
TTGACGC14000.0122.598611
TAGCGTT22250.0122.425731
TCGGCGC5800.0122.204961
Produced by FastQC (version 0.11.2)