FastQCFastQC Report
Sun 15 Jan 2017
HH75LAFXX_n01_tpq1ss_14_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH75LAFXX_n01_tpq1ss_14_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8050552
Sequences flagged as poor quality0
Sequence length145
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTTGATGGAAGCAAGTACTTTTTAGATACCCAGTAAAAGTCTCCATAT634470.7881074490295822No Hit
TATGAACAACAATTGTTGTAGTCGCAACTACGGTAATTGGTCCTAAAAAA312210.3878119164996388No Hit
TTGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAA224110.27837842672154656No Hit
TCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCACATTCT213060.2646526598424555No Hit
TGTGCTAGATCTCTTCTTTGATGGAAGAATATTAGATTCAGATATGTTGG192710.2393748900696499No Hit
TTGAGAAAAGAAATTATACATTTCAGAAACGTCAATAGGGTATCATCATC183220.2275868785146658No Hit
TCTCTGGAACAGCTGATGAAGCAGGTGTTGTTGTCTGTTGAGAGTTAGCC160460.19931552519628468No Hit
TGAAGAAATTAAAAGAATTTCTACTAAATCCCAATTGTTATATATTTGTT157730.19592445337909747No Hit
TCAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTTGGCGT157340.19544001454807075No Hit
TAGTATATGTTGAGCAAGTGTGTTGATGTAGTATAGTAAGTCAAATCTAA155110.1926700181552768No Hit
TGTTATTAGATGTGGATACATTGTGAGCCCTGGCTGTTTTCGATTTCGAA152950.18998697232189793No Hit
TCTTTAAATGGGTCAGGGAATTCGGTAGGAGATTCTGGAGGTAGATCAGG145980.1813291809058559No Hit
TCTTATAAGGGTTCGTGATGCTCCTGAATCGAGAAGGAGGTGTCCAGGGA134100.16657242882227205No Hit
TATTTGCGTGTACTTTCACTTGTATGGACATTATTGATGGTGGGTACGGA129600.16098275000273274No Hit
TCTTATGTATAATTCTTCTTTGATGTCTGCATACAAATATGCCGAAGATA126760.15745504159217902No Hit
TCCCTGTTGTTCTTCATAAATAGCATGGATATCTAAGAACAGTTCAGCGA125880.15636194884524687No Hit
TGCCTTCCATTGCCTCCTTTTTTTCTCTTCCAGAACTCTCTCAGCAGTAT124940.15519432704738756No Hit
TGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAG119110.14795258759896215No Hit
TGCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCT117150.14551797193534058No Hit
TAAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCCCT112360.13956806936965316No Hit
TTTCATCATCAGTGATCTGACGTACGGGTTTTCCGTTTACTGTCGGAATA112300.139493540318726No Hit
TCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCTC108900.1352702274328518No Hit
TTGAAATTGTAGGTCACCAATAGCGTTAATTGGTATATTTCTTTTTTGAG107890.13401565507557742No Hit
TGCCTCGATATATTTTTCTTTTTCTTTAATATCTTTATTATAGGTGATTG106530.13232632992122775No Hit
TTTCTTTAAATGGGTCAGGGAATTCGGTAGGAGATTCTGGAGGTAGATCA105690.13128292320824708No Hit
TATGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTG105090.13053763269897517No Hit
TATTAGATGTGGATACATTGTGAGCCCTGGCTGTTTTCGATTTCGAATTA102680.12754404915339967No Hit
TGGTATGAACAACAATTGTTGTAGTCGCAACTACGGTAATTGGTCCTAAA101870.12653790696588257No Hit
TGTATATGTTGAGCAAGTGTGTTGATGTAGTATAGTAAGTCAAATCTAAA95190.11824033929598865No Hit
TTGCTAGATCTCTTCTTTGATGGAAGAATATTAGATTCAGATATGTTGGG93210.11578088061539134No Hit
TCAAATATTTGAAAAGTGTTATACAAGAAGGTGAGTTCATCATCAGTGAT90960.11298604120562167No Hit
TCATCGATTGAAGGTGAACGGTGTATAATCCTTTTCTCAGTCTCTTGGTC89090.1106632191183909No Hit
TTGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTGG86160.10702371713144639No Hit
TATGAGAAAAGAAATTATACATTTCAGAAACGTCAATAGGGTATCATCAT83690.1039556045349437No Hit
TAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAGGAG82810.10286251178801156No Hit
TAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTTGGCGTT82170.10206753524478818No Hit
TCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAACTACAAATGC80950.10055211120926863No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGCC10750.074.336371
TGTCCGC16000.067.751131
TCCCGCA28450.060.084821
TCGCGGC9450.057.355461
CCAGTCC59450.056.1067124
CAGTCCA60150.055.1071785
TGCCCGC47250.054.2670861
TGCCCGT30650.053.958321
TCGGGTC25500.053.9556541
TGCGGCT27400.053.7647061
TCGCCGG16050.053.6856961
TCGGCGC10200.050.4131131
GGAAGCA186100.050.22169
TCGCGCG4300.050.0962451
TCGGCCG11200.049.01411
GCCCGCA46100.048.8380972
TCGGGCT17150.048.6215821
TCGCCCG13200.048.431271
TGGCCCC26600.046.4997181
TCCAAGC75300.045.7727248
Produced by FastQC (version 0.11.2)