FastQCFastQC Report
Sun 15 Jan 2017
HH75LAFXX_n01_tpq1ss_13_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH75LAFXX_n01_tpq1ss_13_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1511458
Sequences flagged as poor quality0
Sequence length145
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGAACAACAATTGTTGTAGTCGCAACTACGGTAATTGGTCCTAAAAAA121300.8025363589328979No Hit
TATTTGATGGAAGCAAGTACTTTTTAGATACCCAGTAAAAGTCTCCATAT111790.7396169791022972No Hit
TCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCACATTCT50530.3343129613922451No Hit
TTGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAA45170.2988505138746826No Hit
TAAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCCCT44890.29699799795958604No Hit
TGTGGGTAGCAATTGCGGGCTGCAGTCTATCTGAGATGGGCCGTTGTGGC43030.2846919993807304No Hit
TGTGCTAGATCTCTTCTTTGATGGAAGAATATTAGATTCAGATATGTTGG37340.24704622953466124No Hit
TCCTAAGCGCAGCATATCATGAAAGAGCGGTGAGACATGCTTGGAAGGGG36060.2385775853513627No Hit
TCTTTAAATGGGTCAGGGAATTCGGTAGGAGATTCTGGAGGTAGATCAGG35840.23712203713235827No Hit
TCTAAGCGCAGCATATCATGAAAGAGCGGTGAGACATGCTTGGAAGGGGA35510.23493871480385164No Hit
TCTCTGGAACAGCTGATGAAGCAGGTGTTGTTGTCTGTTGAGAGTTAGCC35390.23414477941166742No Hit
TTGAAATTGTAGGTCACCAATAGCGTTAATTGGTATATTTCTTTTTTGAG35120.23235842477925286No Hit
TCTTATGTATAATTCTTCTTTGATGTCTGCATACAAATATGCCGAAGATA32230.2132378140841492No Hit
TCAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTTGGCGT31710.20979742738468418No Hit
TATTTGCGTGTACTTTCACTTGTATGGACATTATTGATGGTGGGTACGGA29600.19583739673877804No Hit
TCTTATAAGGGTTCGTGATGCTCCTGAATCGAGAAGGAGGTGTCCAGGGA28750.1902136877108064No Hit
TGAAGAAATTAAAAGAATTTCTACTAAATCCCAATTGTTATATATTTGTT28120.18604552690183915No Hit
TATGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTG27760.18366372072528644No Hit
ACGCCAGAATACGAAAGACCAGGTATATGCACAAAATGAGATGCTTGCTT27740.18353139815992242No Hit
TCATCGATTGAAGGTGAACGGTGTATAATCCTTTTCTCAGTCTCTTGGTC27550.18227433378896402No Hit
TGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAG26600.17598901193417216No Hit
TTGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTGG25700.1700344964927904No Hit
TCTCTAAAGAAGAGCGGTCACCGAGAGTACTAACGATGGGTTCGTAAGCG23310.15422194993178773No Hit
TGTTATTAGATGTGGATACATTGTGAGCCCTGGCTGTTTTCGATTTCGAA22900.15150933734182492No Hit
TAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCCCTTG22840.1511123696457328No Hit
AAAAGTAAAAATGTCTACAGTAGAGTCAATAGCAAGGCCACGACGCAATG22710.15025227297086655No Hit
TGCCTTCCATTGCCTCCTTTTTTTCTCTTCCAGAACTCTCTCAGCAGTAT22620.14965682142672837No Hit
ATCCACTGTTCACCATAAACGTGACGATGAGGGACATAAAAAGTAAAAAT21670.1433714995719365No Hit
TAGTATATGTTGAGCAAGTGTGTTGATGTAGTATAGTAAGTCAAATCTAA21340.14118817724342986No Hit
AGTGAGGTGATTTATGTTTGGTGCTATTGCTGGCGGTATTGCTTCTGCTC20790.13754930669591878No Hit
TAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAGGAG20330.13450588769254587No Hit
TCTGTGATTGTGGCCCAGGTGGAAAGTACATAGGCGACATTTGATAAGGT20250.1339765974310897No Hit
TTTCTTTAAATGGGTCAGGGAATTCGGTAGGAGATTCTGGAGGTAGATCA20230.1338442748657257No Hit
TTGTTAACGTATTTAGCCACATAGAAACCAACAGCCATATAACTGGTAGC20200.13364579101767962No Hit
TTGAGAAAAGAAATTATACATTTCAGAAACGTCAATAGGGTATCATCATC19890.131594791254537No Hit
TGCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCT19820.13113166227576287No Hit
TCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCTC19800.13099933971039884No Hit
AGTGGCTGGAGACAAATAATCTCTTTAATAACCTGATTCAGCGAAACCAA19790.13093317842771682No Hit
TTGCTAGATCTCTTCTTTGATGGAAGAATATTAGATTCAGATATGTTGGG19490.12894833994725624No Hit
TATTAGATGTGGATACATTGTGAGCCCTGGCTGTTTTCGATTTCGAATTA19370.128154404555072No Hit
TCAGAAACGGCAGAAGTGCCAGCCTGCAACGTACCTTCAAGAAGTCCTTT19000.12570643709583726No Hit
TGGCGAGAAATAAAAGTCTGAAACATGATTAAACTCCTAAGCAGAAAACC18800.12438321144219687No Hit
TTGCTACTTTCACGCACAGTAGGGTTGAGTCTGATGCACCACTGACATTG18650.12339079220196657No Hit
TCTCCCGTAACACTATCTCTGATACCCTACCCTTTTCTTGCAAACCGCCA18320.12120746987345993No Hit
TGTATATGTTGAGCAAGTGTGTTGATGTAGTATAGTAAGTCAAATCTAAA18000.11909030882763531No Hit
TTTTCATTTTTACCTATTTTTTCAGAGTACCACTCCCAAACTTCAGCAGC17640.11670850265108258No Hit
AATCGAAGTGGACTGCTGGCGGAAAATGAGAAAATTCGACCTATCCTTGC17430.11531911571476018No Hit
TGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCCCTTGC17370.11492214801866807No Hit
TAGCGATGATAGATTCCTCGGTATTCTGTTCAGAAACAGTAGTTGGCGTT16850.11148176131920305No Hit
TACCACATCTATTGACATTATGGGTCTGCAAGCTGCTTATGCTAATTTGC16680.11035701951360871No Hit
TTTCATCATCAGTGATCTGACGTACGGGTTTTCCGTTTACTGTCGGAATA16540.10943076155606044No Hit
TGTAACAGTGAATGTAGCGCCATTCGTTACGAAAGTCGTTGGTTCTGGGC16180.10704895537950773No Hit
TCTCGTTGGCACTAGTGAGTTTATACCTGCATTAGCCAGGTAGCAATAGT16060.10625501998732349No Hit
TCCTTGCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCGCCATCAACT15960.1055934071605033No Hit
TGTTTGGTGCTATTGCTGGCGGTATTGCTTCTGCTCTTGCTGGTGGCGCC15670.10367472996272473No Hit
TGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAGGAGTTT15660.1036085686800427No Hit
TCAGAAATATCCGAAAGTGTTAACTTCTGCGTCATGGAAGCGATAAAACT15430.10208685917835625No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGCC1250.088.94751
TATGAAC25150.072.944031
TAGACGG5950.068.906291
TCCTAAG6800.065.402581
GGCCCGC852.4738256E-1065.402582
TCGCTCG1001.4551915E-1162.5412141
TCCCGCA5150.062.068961
TGCGCGC904.110916E-1061.7691041
CGAGGCT2500.061.153439
GGGTAGC9600.060.8059734
TCCGGCC1950.060.5812341
TGCGGCT4050.060.0532951
TCGAGGC2700.059.197358
TGCCCGT5100.058.5898131
TCGGTCG2200.056.8556481
TGCCGGG2450.056.7267271
ACAATTG31950.056.5510529
TCGGGCT2100.056.2540051
CAGTCCA15000.055.5940365
CTCCCGT8650.053.824812
Produced by FastQC (version 0.11.2)