FastQCFastQC Report
Sun 15 Jan 2017
HH75LAFXX_n01_tpq1ss_12_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH75LAFXX_n01_tpq1ss_12_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3054617
Sequences flagged as poor quality0
Sequence length145
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAA290310.950397382061319No Hit
TATGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTG195600.6403421443670353No Hit
TTGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTGG183930.6021376820727443No Hit
TCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAACTACAAATGC175860.5757186580183374No Hit
TAGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAAGA162330.5314250526334399No Hit
TCATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAACTACAAA156680.5129284620625106No Hit
TTAGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAAG136220.4459478880658361No Hit
TAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAGGAG130230.4263382283278067No Hit
TATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAACTACAAAT123780.40522265148134773No Hit
TCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCACATTCT113450.37140499119856923No Hit
TGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAG109620.35886659440447033No Hit
TAGTGAGGGCACTATATGGGTGTGATTTTTCATCACATACAATGGCTGAT106690.3492745571703425No Hit
TCGATGGTCTTGGGGCTCAGTACTTAAGTAGGTCTTGTGCTACTCTTGTA104030.34056642780420593No Hit
TCCTAAGCGCAGCATATCATGAAAGAGCGGTGAGACATGCTTGGAAGGGG102630.33598320182202873No Hit
TAAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCCCT94320.30877848188496304No Hit
TGTCAGTCAGGACCATAGCTACTGGCAATTATCGAGATGCTGCTGAAAGA88370.2892997714607101No Hit
TGCCTTCCATTGCCTCCTTTTTTTCTCTTCCAGAACTCTCTCAGCAGTAT86790.2841272735665388No Hit
TCTAAGCGCAGCATATCATGAAAGAGCGGTGAGACATGCTTGGAAGGGGA82300.2694282130951278No Hit
TTGCTACTTTCACGCACAGTAGGGTTGAGTCTGATGCACCACTGACATTG74040.24238717980028263No Hit
TCTCCCGTAACACTATCTCTGATACCCTACCCTTTTCTTGCAAACCGCCA73240.23976819352475287No Hit
TGCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCT68450.2240870132000182No Hit
TGTTGACATTGAGAATATAGATAGTTATAAGAAGACAAGGATGATTGCCA63680.20847130753217177No Hit
TGCCGGCTGTGCTCGCGCATTTATCCGGTGCATAGCGTCCATTATTCTTA60990.19966496618070284No Hit
TGTAGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAA60300.19740609051805838No Hit
TCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCTC59160.19367403507542846No Hit
TTCAGTCAGGACCATAGCTACTGGCAATTATCGAGATGCTGCTGAAAGAT58470.191415159412784No Hit
TAGCCGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCT56010.18336177661552988No Hit
TAAAGTGAGGGCACTATATGGGTGTGATTTTTCATCACATACAATGGCTG55600.18201954614932084No Hit
TGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTGGG55080.1803172050702265No Hit
TCTCTAAAGAAGAGCGGTCACCGAGAGTACTAACGATGGGTTCGTAAGCG52960.1733768914400725No Hit
TATGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCA51310.1679752322467923No Hit
TCTGGCCTTGAAACAACAGATGCAGTCATGGCTCTTCTCTTTTCCCAGCA49870.1632610569508387No Hit
TCAGAAAGCTAAAAAAAAGAAAATAGAAAACAACCAGAAGAAAACAAAAA48330.15821950837044382No Hit
TAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAGGA47790.1564516926344612No Hit
TGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAGGAGTTT46910.15357080773137843No Hit
TCTCGTTGGCACTAGTGAGTTTATACCTGCATTAGCCAGGTAGCAATAGT45540.14908579373453365No Hit
TGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAAGAA45130.14774356326832463No Hit
TAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCCCTTG44370.14525552630657132No Hit
TACAATGTACAAAGCCTATAAGGGTTTGGCAGTCTCATACTATGCTAACC43490.14237464140348857No Hit
TACAGAAAGCTAAAAAAAAGAAAATAGAAAACAACCAGAAGAAAACAAAA43240.14155620819238549No Hit
TTTCGTTCAGGCCGCACTAGCATGTTGTACCGGACAAGATGCGCCCCTAA42810.14014850306928822No Hit
TGACGAACTTCCACCGGATATAGAATAGGTGTCGAAGACGATGAGGACTT42310.1385116366470821No Hit
TCGCCGGCTGTGCTCGCGCATTTATCCGGTGCATAGCGTCCATTATTCTT41070.13445220792001092No Hit
TGTGAGGGCACTATATGGGTGTGATTTTTCATCACATACAATGGCTGATT40620.1329790281400254No Hit
TAACAGAAAGCTAAAAAAAAGAAAATAGAAAACAACCAGAAGAAAACAAA39200.12833032750096002No Hit
TGGCCTTGAAACAACAGATGCAGTCATGGCTCTTCTCTTTTCCCAGCAAA38660.1265625117649774No Hit
TGGGCCCCAAAAATCAGTTTAACCCAAAAATCCCCAAAGGAATCAAGGGA38460.12590776519609495No Hit
TATGAAATTAGCTGGCGTATTTGATCTGGATGACGTTCAAGACTCGGTAC38290.12535123061254488No Hit
TTGAGAATATAGATAGTTATAAGAAGACAAGGATGATTGCCAAACTGATT37220.12184833646902377No Hit
TCTCTGGAACAGCTGATGAAGCAGGTGTTGTTGTCTGTTGAGAGTTAGCC37170.12168464982680316No Hit
TGCGCCATAAAATATTGTAATTACTTCCAAAGGAAAAAACTACAAATGCA36370.11906566355127336No Hit
TTTACTACCAGAATATTTTAAACCTAAAACTAATTTGAATATCAACTCTT36320.11890197690905276No Hit
TCGTTCGTTCAGGCCGCACTAGCATGTTGTACCGGACAAGATGCGCCCCT35810.11723237315840251No Hit
TAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCCCTT35280.11549729475086401No Hit
TATGAACAACAATTGTTGTAGTCGCAACTACGGTAATTGGTCCTAAAAAA35210.11526813345175517No Hit
TCCCTTTAGCAAACCTCCATTTATATCCTGCGAGAAGAGCTCCTTCTCCT35130.11500623482420219No Hit
TTCAGTACTTAAGTAGGTCTTGTGCTACTCTTGTACACAGTAGGATTGAG34990.11454791222598447No Hit
TCATCGATTGAAGGTGAACGGTGTATAATCCTTTTCTCAGTCTCTTGGTC34860.11412232695621087No Hit
TCTCCAGCGCTATATTGGCCCTGTTCAGTCAATACTTGTAAGTGGTAAGC34780.11386042832865789No Hit
TCGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAGGAGTT34440.11274735916155774No Hit
TCGGGTCAAGATCATCGCGCCATAAAATATTGTAATTACTTCCAAAGGAA34220.11202713793578703No Hit
TGCCTCCTTTTTTTCTCTTCCAGAACTCTCTCAGCAGTATTCTCACAAAC33720.11039027151358093No Hit
TGTTCCAAAAAATATGGCAAGCCCACAGTAACGGACAGCTTTACCTGTTT32390.10603620683051264No Hit
TCCCGTAACACTATCTCTGATACCCTACCCTTTTCTTGCAAACCGCCACA31730.10387554315320056No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTAAG13150.0103.574941
TCGGGTC8750.093.712671
TCGGGAC5300.089.157411
TTAGGAG21400.087.674721
TCTAAGC13550.086.67061
TGGGCCC11250.082.1530151
TCGGCCG4350.079.873951
TGCCGGC17600.078.571461
TCGGGCC3900.078.3993451
TCCCGCA16000.075.570741
TCGGCGC2450.073.744841
TCGGCGT6100.071.768711
TCGGGCT6300.071.696381
TGGCCCC9650.071.29061
GTCAGTC17400.070.6884462
CGTCCGG4750.070.221811
TAAACGT11500.069.490331
TGCCCGT6500.067.352171
TATCGCG28700.066.100561
TGCCGGG4600.064.958361
Produced by FastQC (version 0.11.2)