FastQCFastQC Report
Sun 15 Jan 2017
HH75LAFXX_n01_tpq1ss_11_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH75LAFXX_n01_tpq1ss_11_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3510929
Sequences flagged as poor quality0
Sequence length145
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACGAACTTCCACCGGATATAGAATAGGTGTCGAAGACGATGAGGACTT264040.752051664958192No Hit
TAGTGAGGGCACTATATGGGTGTGATTTTTCATCACATACAATGGCTGAT239040.6808454400530458No Hit
TAAAGTGAGGGCACTATATGGGTGTGATTTTTCATCACATACAATGGCTG153950.4384879329658902No Hit
TAAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCCCT146830.41820840011290455No Hit
TGTCAGTCAGGACCATAGCTACTGGCAATTATCGAGATGCTGCTGAAAGA133020.3788740814753018No Hit
TGGACGAACTTCCACCGGATATAGAATAGGTGTCGAAGACGATGAGGACT114160.3251561054068596No Hit
TCGATGGTCTTGGGGCTCAGTACTTAAGTAGGTCTTGTGCTACTCTTGTA113830.32421618323811163No Hit
TGTGAGGGCACTATATGGGTGTGATTTTTCATCACATACAATGGCTGATT100130.2851951719900915No Hit
TTGCTACTTTCACGCACAGTAGGGTTGAGTCTGATGCACCACTGACATTG94420.2689316702217561No Hit
TATGAAATTAGCTGGCGTATTTGATCTGGATGACGTTCAAGACTCGGTAC88640.2524687910236863No Hit
TGCCTTCCATTGCCTCCTTTTTTTCTCTTCCAGAACTCTCTCAGCAGTAT88390.25175672877463484No Hit
TTGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAA87860.2502471568066458No Hit
TGCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCT86630.24674381054131259No Hit
TATGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTG86590.24662988058146434No Hit
TGTAGCAATGTCAGTGATCATAGCAGTGGTTTCAGAAATGTTGGCAGACA81260.23144871343168716No Hit
TAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCCCTTG79230.2256667679693893No Hit
TATATGAAATTAGCTGGCGTATTTGATCTGGATGACGTTCAAGACTCGGT78490.22355906371219697No Hit
TCTCCCGTAACACTATCTCTGATACCCTACCCTTTTCTTGCAAACCGCCA76690.21843221551902645No Hit
TCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCTC63630.18123408362857807No Hit
TGCCGGCTGTGCTCGCGCATTTATCCGGTGCATAGCGTCCATTATTCTTA63400.18057898635945072No Hit
TAGCCGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCT63180.17995237158028543No Hit
TGAGCAGTTGTAATGCCAGGTGGATCGGTCCACAGTCAACATCCAGTCGA62510.1780440447528275No Hit
TAGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAAGA61220.17436980354772197No Hit
TTGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTGG60220.17152155455151613No Hit
TAGCAGTTGTAATGCCAGGTGGATCGGTCCACAGTCAACATCCAGTCGAA59860.17049618491288202No Hit
TAAGTGAGGGCACTATATGGGTGTGATTTTTCATCACATACAATGGCTGA55400.15779299438980393No Hit
TAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCCCTT54120.15414723567466046No Hit
TTGATCTTGGAGATCGCGTCTGGGTTGAATTGTGCAGTGGACACGAGAGG52970.15087174932902372No Hit
TGGAAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCC50200.14298209960953354No Hit
TAGAGAAACGCAACAGGACAGATATCAAGACTGTTAACATAAGTTATGCA50200.14298209960953354No Hit
TTCAGTCAGGACCATAGCTACTGGCAATTATCGAGATGCTGCTGAAAGAT50030.14249789728017853No Hit
TTAGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAAG49160.14001992065347946No Hit
TGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCCCTTGC49000.13956420081408652No Hit
TGAAGAAATTAAAAGAATTTCTACTAAATCCCAATTGTTATATATTTGTT48930.1393648233843521No Hit
TCTCTAAAGAAGAGCGGTCACCGAGAGTACTAACGATGGGTTCGTAAGCG48730.13879517358511095No Hit
TTTGCTACTTTCACGCACAGTAGGGTTGAGTCTGATGCACCACTGACATT48700.13870972611522478No Hit
TGGTAGCAATGTCAGTGATCATAGCAGTGGTTTCAGAAATGTTGGCAGAC48670.13862427864533858No Hit
TCTACGAAGTACTCTGATAACTAACTTTGCAATGTTCAGGTGCGAGGATG48150.13714318916731155No Hit
TCTCGTTGGCACTAGTGAGTTTATACCTGCATTAGCCAGGTAGCAATAGT47910.13645960940822213No Hit
TTATGAAATTAGCTGGCGTATTTGATCTGGATGACGTTCAAGACTCGGTA47390.1349785199301951No Hit
TTCAGTACTTAAGTAGGTCTTGTGCTACTCTTGTACACAGTAGGATTGAG47180.1343803876409919No Hit
TCTCCCCCCTATGGAAGATCCACTGCTGCGCCAGCCCTTGCTGTGTGAAA42660.12150630217814146No Hit
TGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAG42110.11993976523022823No Hit
TCATTTGTCGTCGCTGTTACACCCCGTTGGGCAGCTACATGATTTTTGGC41920.11939859792094913No Hit
TGCTTATCCCGACATGCTGTTTTACCATTCAGTGGCAATGATTGAGAGCT41910.11937011543098708No Hit
TGTCTCCCCCCTATGGAAGATCCACTGCTGCGCCAGCCCTTGCTGTGTGA41750.11891439559159414No Hit
TCCGGTTCGCAATACTACAAGCTTTGATAAGCACGTGTGTTGCTTCTAAA40890.11646490145485712No Hit
TGCCTCCATCACGGGTCACCTTTGCTGACGCTGGATAAAACTCCCCTAAC40580.11558194426603328No Hit
TCTGGCCTTGAAACAACAGATGCAGTCATGGCTCTTCTCTTTTCCCAGCA40210.11452809213743714No Hit
TGCCCTCACTTTTCCCCACTCGTATTTCGTTGAAGTGTAAGCGTGTATTT39630.11287610771963774No Hit
TCTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCCCAGGACA39070.11128108828176246No Hit
TGAGAAACGCAACAGGACAGATATCAAGACTGTTAACATAAGTTATGCAA38960.11096778089217982No Hit
TATTTGTCGTCGCTGTTACACCCCGTTGGGCAGCTACATGATTTTTGGCA38330.10917338402457012No Hit
TACGAACTTCCACCGGATATAGAATAGGTGTCGAAGACGATGAGGACTTA38080.10846132177551868No Hit
TGACAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGACCAAA37440.10663844241794694No Hit
TATCCGGTTCGCAATACTACAAGCTTTGATAAGCACGTGTGTTGCTTCTA36370.10359081599200666No Hit
TAAGAGAAACGCAACAGGACAGATATCAAGACTGTTAACATAAGTTATGC35820.10202427904409345No Hit
TGTGATAACAACCTTCTTGGCACCAGCGTCAATGTGCTTTTGAGCAGTGT35770.10188186659428317No Hit
TATTGCTACTTTCACGCACAGTAGGGTTGAGTCTGATGCACCACTGACAT35430.10091346193557317No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAGTC26500.079.978312
TGCCGGC20900.078.133961
TCCCGCA13150.077.151681
TGGACGA21150.076.224731
TCGGGAC5650.071.3342061
TCGGGCC4900.070.907511
TAGACGG16100.069.057741
TAAGACG11900.067.737521
TAAAGTG31850.066.107611
GACGAAC58950.065.65832
ACGAACT60050.064.338933
TGACGAA61900.063.8763241
TCGGCCG5800.063.4988941
GTCCCCC5700.063.39382
GTCTCCC13600.062.8469922
TAGTGAG63200.059.9235731
TAAAGAC34850.058.42291
TGCCCGC27250.058.3965571
TGTCCCC12600.057.9077951
AGTCAGG37300.057.194465
Produced by FastQC (version 0.11.2)