FastQCFastQC Report
Sun 15 Jan 2017
HH75LAFXX_n01_tpq1ss_10_umi.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH75LAFXX_n01_tpq1ss_10_umi.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3560606
Sequences flagged as poor quality0
Sequence length145
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCCCT701321.9696647143772716No Hit
TAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCCCTT201420.5656902223947272No Hit
TAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCCCTTG174850.49106809346498886No Hit
TCCTAAGCGCAGCATATCATGAAAGAGCGGTGAGACATGCTTGGAAGGGG174380.48974809344252074No Hit
TGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCCCTTGC173160.4863217104054759No Hit
TCTCCAGCGCTATATTGGCCCTGTTCAGTCAATACTTGTAAGTGGTAAGC127190.35721447416535274No Hit
TCTCGTTGGCACTAGTGAGTTTATACCTGCATTAGCCAGGTAGCAATAGT127010.35670894224185434No Hit
TCTCCAGTCCAAGCCAATAATAAATATATTGCAACACCAATCCACATTCT115370.32401787785562347No Hit
TCTAAGCGCAGCATATCATGAAAGAGCGGTGAGACATGCTTGGAAGGGGA104590.29374213265944055No Hit
TCGATGGTCTTGGGGCTCAGTACTTAAGTAGGTCTTGTGCTACTCTTGTA97690.2743634089253346No Hit
TTGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAA88750.24925532339157996No Hit
TAGCCGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCT87970.24706468505642018No Hit
TATGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTG87290.2451548977898706No Hit
TTGCTACTTTCACGCACAGTAGGGTTGAGTCTGATGCACCACTGACATTG84170.2363923444492314No Hit
TCTCTGGAACAGCTGATGAAGCAGGTGTTGTTGTCTGTTGAGAGTTAGCC81470.22880936559675516No Hit
TGTCAGTCAGGACCATAGCTACTGGCAATTATCGAGATGCTGCTGAAAGA75790.21285702489969405No Hit
TGCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCT75480.21198638658700233No Hit
TGCATGATGGGGGTTCTCTGGAACAGCTGATGAAGCAGGTGTTGTTGTCT74500.20923404611462204No Hit
TTGATGTTACTGCTACTGATTTAGTTACAGGTAGAAAAGTGTCGAATTGG72330.20313957792578002No Hit
TCTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCCCAGGACA71470.20072425873573205No Hit
TGTTCCAAAAAATATGGCAAGCCCACAGTAACGGACAGCTTTACCTGTTT66960.18805787554141065No Hit
TAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATC66470.18668170530522052No Hit
TGCCGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTG65940.1851931946415863No Hit
TCCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCTC64900.1822723435280399No Hit
TAGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAAGA61750.1734255348668176No Hit
TGCGAGTAAATCCTGATGGCACCGCCAAATTAAACCGCCGAAGCGATCAA59450.1669659602887823No Hit
TCTCTAAAGAAGAGCGGTCACCGAGAGTACTAACGATGGGTTCGTAAGCG55680.1563778750021766No Hit
TGGAAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCC53690.150788938736833No Hit
TGAAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCCC53420.15003064085158538No Hit
TCGACGGAGGAAAGACGGTTGCCACAACAGGGCACGGTGCGGAATAGAGA53320.14974978978297515No Hit
TGTAGAGCGTAGCCTGGGATGCCACGAGGATGTATTTTGGAGTTAGGGTT52760.14817702379875786No Hit
TGCCGGCTGTGCTCGCGCATTTATCCGGTGCATAGCGTCCATTATTCTTA51730.14528425779207246No Hit
TCAGAAATGATCAACACCAGGCGCCCAGCAACGTTCAATCTATCTACGCT50740.1425038322128312No Hit
TGTGCTAGATCTCTTCTTTGATGGAAGAATATTAGATTCAGATATGTTGG50550.14197021518247174No Hit
TGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAG50350.1414085130452513No Hit
TACTCGATCCAGCACGGGAAAGAGCAATCGTCATAACTATGGTTTAGATT49750.1397234066335899No Hit
TAGTGAGGGCACTATATGGGTGTGATTTTTCATCACATACAATGGCTGAT49220.1382348959699557No Hit
TCGGGACACTTCAGAACGGACTCTCCCGCCTCCGGGCAGACAGCTCTGCC47740.13407830015452427No Hit
TCGTTCGTTCAGGCCGCACTAGCATGTTGTACCGGACAAGATGCGCCCCT46910.13174723628505935No Hit
TGGCATGATGGGGGTTCTCTGGAACAGCTGATGAAGCAGGTGTTGTTGTC46700.13115744904097787No Hit
TCACTAAAAGAAGCACCTGTGAGAAGCGCCCAGAACAGTATGAGGACGCT45430.12759064046962793No Hit
TCCGCAAAGATCGATTTATTATAGCCGGGACACTTCAGAACGGACTCTCC45200.1269446830118244No Hit
TTAGGAGCACTATAGCTCTGAAACGTAAGGCTAGGATAACATCTAAGAAG44410.12472595956980358No Hit
TCATCGATTGAAGGTGAACGGTGTATAATCCTTTTCTCAGTCTCTTGGTC43660.12261957655522683No Hit
TCAAGGCTCCACTGTGTTCCACCGAATTTCCCACCTGATAATATCTGATA43540.12228255527289456No Hit
TGCCTCCATCACGGGTCACCTTTGCTGACGCTGGATAAAACTCCCCTAAC42790.12017617225831781No Hit
TCTCCCGCCTCCGGGCAGACAGCTCTGCCCAGGACAAATTTACGACGGAG42740.1200357467240127No Hit
TGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCCCTTGCCCAA42520.11941787437307019No Hit
TGTACCCCTTTTTGTTATCAGGAGATGCAGCGTTGATTCAGGCTACAGCC42230.11860340627410054No Hit
TACAAGGCTCCACTGTGTTCCACCGAATTTCCCACCTGATAATATCTGAT41270.11590723601544231No Hit
TCTCCCGTAACACTATCTCTGATACCCTACCCTTTTCTTGCAAACCGCCA40290.11315489554306205No Hit
TTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCCCTTGCCCAAAG40170.11281787426072978No Hit
TAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCAAGGAG39010.10956000186485108No Hit
TGCGCCATCTAGATGAATACGTGAGGCCCATTGGAGGCTCCTTGAGACTC38660.10857702312471529No Hit
TCTTTAAATGGGTCAGGGAATTCGGTAGGAGATTCTGGAGGTAGATCAGG38450.10798723588063382No Hit
TATGGAAACGTCAGCAAACACGCCTTCCGCGCCGTATGTGTGTGTGACCA37830.10624595925525038No Hit
TTCAGTCAGGACCATAGCTACTGGCAATTATCGAGATGCTGCTGAAAGAT37190.10444851241614489No Hit
TGACGAACTTCCACCGGATATAGAATAGGTGTCGAAGACGATGAGGACTT36430.10231404429470714No Hit
TGGTTGCCACAACAGGGCACGGTGCGGAATAGAGAACTATCCCTTGCCCA36110.10141532087515438No Hit
TTTCGTTCAGGCCGCACTAGCATGTTGTACCGGACAAGATGCGCCCCTAA35810.10057276766932371No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTAAG22550.0120.492141
TAAAGAC96600.0105.891041
TCGGGAC8400.0104.2364961
TAGACGG26250.098.213931
TAAGACG27400.098.149681
TAACGCA15400.083.930681
TCTAAGC20700.081.576381
TGCCGGG12900.075.955271
CCTAAGC36550.074.146992
CGCCGGG9200.073.265622
TCGGGCC3600.069.490991
TCTCGTT32100.067.3261641
TGCCGGC18750.065.228881
TGGCTCC12100.063.7479361
GGCTCCG9450.063.23872
TCGCCGG20050.062.039341
GTCAGTC16400.061.4384842
TCGGGCT6300.060.6667371
TCGCAGC9550.058.9400021
TCGTTCG11450.058.263191
Produced by FastQC (version 0.11.2)