FastQCFastQC Report
Sat 30 Jun 2018
HH5HLBGX7_n03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5HLBGX7_n03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518676304
Sequences flagged as poor quality0
Sequence length99
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37052600.7143684744078843No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG14769310.28475004325626563No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCA8199380.15808279531505262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT17187950.071.404991
AGCAGTG17226650.071.0135042
CAGTGGT18500050.065.9535454
GCAGTGG19091600.064.1193163
AGTGGTA19825450.061.7606965
TGGTATC20388550.059.8293467
GTGGTAT20446450.059.8225986
GGTATCA20585600.059.353698
GTATCAA25101650.048.558639
CATGGGG12789350.033.5807724-25
AGAGTAC20332200.033.13189718-19
GCAGAGT20582100.032.7748816-17
TACATGG20389400.032.68193422-23
CAACGCA21030100.032.02299512-13
ATCAACG21344900.031.60858510-11
ACGCAGA21574800.031.24576814-15
CAGAGTA20491700.029.8531918-19
ACATGGG20254750.029.76414324-25
CGCAGAG20967750.029.30043216-17
GAGTACA21123250.028.9158620-21
Produced by FastQC (version 0.11.2)