FastQCFastQC Report
Sat 30 Jun 2018
HH5HLBGX7_n02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5HLBGX7_n02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518676304
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGCCG7533477814.524430250432264No Hit
TTCCCGAC7354269914.178920153637865No Hit
CCCACAGT6229423212.010232879271847No Hit
GCAGTAGA6025742011.61753863349809No Hit
AGTAGTCT5894522011.364548475690533No Hit
CAGTACTG5517594710.637838392555524No Hit
TATGATTC492615489.497551289715368No Hit
ATGCTGAA455547358.782883399277095No Hit
GGGGGGGG189889523.661040971711713No Hit
TTCCCGCC8901550.17162052577593753No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers