FastQCFastQC Report
Sat 30 Jun 2018
HH5HLBGX7_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5HLBGX7_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences518676304
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAATG1246650.017.8304791
CTGCGGA1187700.017.815121
GACTAAC1250750.017.71661
CAATGGA1333650.017.6461938
TGACTAG1469250.017.529861
ACTGAAC1371050.017.4591981
GAATGAA1270800.017.4279841
CTCGAAA1330050.017.3853191
TTGAACG1316650.017.3643971
GATGAAA1368600.017.3292711
ATGCGAT1417100.017.289161
CCTACCA1528050.017.2189581
ATAGACC1316300.017.1998331
CGCTGGA1197200.017.186791
GGGACCT1075200.017.1233881
GACCTGG1315000.017.10581
GGATTAC1449800.016.9470271
TGACGGC1070950.016.9196471
ACTGATG1076200.016.911291
AGAGCGA1521950.016.8828281
Produced by FastQC (version 0.11.2)