FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34177597
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30508238.926382390195542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGAGA96600.013.4917524
CGGGGAT25600.08.909951
TTCCCTA31450.08.4376888
CGTGTAT16100.08.39129714-15
GAGCGGG61400.08.2579369
CGGGCAT21350.08.0126951
CCCACAT65300.07.93208361
CCGGTTT17700.07.7857191
CCGAAAT15403.45608E-117.71424251
CCGGAAT17651.8189894E-127.5385411
AAAGGGG89850.07.52289380-81
GGGGGGA256150.07.5206582
CTAGCTC27100.07.51946746
AAAAGGG87200.07.48128178-79
CGGGAAT16351.3278623E-107.2660141
TCCCTAC32650.07.2567629
TCCGATC21200.07.17278961
CTGGGAT71050.07.15638451
GTCGGTT13951.7256752E-87.15350771
CCGATCT21950.07.1335152
Produced by FastQC (version 0.11.5)