FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n02_WT_PR8_M2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n02_WT_PR8_M2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15584961
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG5151.5385012E-812.9060033
ACCTTAC49400.012.1095646
AACCTTA62150.010.6943665
ACATCTA103400.010.3764244
CGGATTA32100.09.3943956-57
CCGGAAT39900.09.1660581
CGCATAT10456.1299943E-109.0903051
AAGGGGG148450.09.00957980-81
CCCGAAT27400.08.8406531
CGGGGAT45250.08.8170941
CCGGGAT48500.08.8138481
ATCTACA114300.08.8059156
TTACTCG34500.08.74260760-61
AAAAGGG150900.08.66266278-79
CGCAAAT18700.08.635791
CATCTAC122550.08.5614465
CCCACAT130900.08.5269361
CGACGAT18450.08.2379351
CGCGTAC11705.120455E-98.1191191
CGGCAAT25300.08.0725861
Produced by FastQC (version 0.11.5)