FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n02_WT_PR8_M1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n02_WT_PR8_M1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17203756
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCTA115650.011.8633414
ACCTTAC49700.011.6545676
GGGGGGA247500.011.5682822
CGGGAAT41950.011.0950231
CGCGTAA4356.762298E-510.9180451
AAGGGGG184200.010.39525780-81
AAAAGGG193200.010.320950578-79
TAGGGGG65000.010.2597450-51
ATCTACA129650.010.2536436
CATCTAC132150.010.1669455
GCCGAAT27450.09.8620371
CCCACAT152850.09.8498121
CGGGGAT53150.09.7399641
CGCAAAT21750.09.607881
GGGGGAG256950.09.4969293
CGCGGAT17650.09.41797351
TACAGAC124800.09.3609649
CGCCGAT28650.09.28319551
AACCTTA66600.08.9106015
CTCGTAT18400.08.7759711
Produced by FastQC (version 0.11.5)