FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n02_WT_PR8_F2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n02_WT_PR8_F2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15792513
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA167350.010.8099122
ACATCTA100350.010.5972134
CGCGAAT9952.3646862E-1110.0242341
ATCTACA116550.09.2877626
CGGGGAT44700.09.24414351
ACCTTAC48450.09.2113346
CCCACAT127150.09.1144071
CGCGGAT16200.09.0886971
CATCTAC124100.08.8371495
CGGAAAT37250.08.7978751
GGCGATT25350.08.6185631
GCGTCGT152100.08.56732594-95
GCCGAAT25000.08.5492391
GTCGGAT23700.08.4169721
CCGGAAT38550.08.3779831
TCGTGTA113250.08.29963794-95
TAGGGGG46450.08.27711950-51
CGCCGAT21250.08.2698521
TACAGAC108800.08.1620959
CGGCGAT19800.08.1558391
Produced by FastQC (version 0.11.5)