FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n02_WT_PR8_F1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n02_WT_PR8_F1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16747055
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATCTA118300.013.40325454
ACCTTAC54550.012.7067316
ATCTACA129200.011.9057916
AACCTTA66500.011.4937185
CATCTAC137150.011.4920795
CGGGAAT38550.010.9654611
CCCACAT154550.010.7562361
TACAGAC130150.010.690759
CGCCGAT23750.09.7992711
AAGGGGG164450.09.69317280-81
AAAAGGG165650.09.58436978-79
CGCGGAT17400.09.2809181
TCTACAG164250.09.25048357
CACATCT189750.09.1321683
CCGGGAT49900.08.9472281
CGGCAAT24550.08.8995421
GGCGAAT23000.08.8797731
CGGGCAT48350.08.8411211
GGGGGGA222800.08.6521792
GCCGGTT34700.08.48641
Produced by FastQC (version 0.11.5)