FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n02_WT_DI222_M2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n02_WT_DI222_M2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15232146
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA205300.012.9282322
ACATCTA105850.012.2441374
ACCTTAC47050.012.1091116
AACCTTA57150.011.1317145
ATCTACA115400.010.8203326
CATCTAC120750.010.1832435
CCCACAT135050.010.1285061
AAAAGGG165100.09.86097678-79
GGGGGAG219800.09.8494213
CCGGGAT48800.09.82991
CGGGGAT50250.09.82980251
TACAGAC114400.09.630619
AAGGGGG164450.09.47615780-81
CGGGTAT16100.09.4399911
CGCGGAT15300.09.0023121
CCGGAAT32200.08.9974921
CGCGAAT9004.2366082E-88.971271
AATCGAG24550.08.702315
GAAATCG42150.08.560313
CGGCAAT22450.08.4623531
Produced by FastQC (version 0.11.5)