FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n02_WT_DI222_M1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n02_WT_DI222_M1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15310275
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA202750.014.378722
ACATCTA106750.011.6960224
ACCTTAC48550.011.2455376
CCCACAT133400.010.8230151
GGGGGAG220900.010.6596253
ATCTACA118950.010.257476
AACCTTA59950.010.1361715
GGGGAGA187600.010.0969524
CATCTAC122750.09.9395065
TAGGGGG57250.09.90783850-51
AAAAGGG165300.09.54714278-79
AAGGGGG163950.09.30231180-81
AAAGGGG177550.09.05806280-81
CGCGAAT7905.644779E-79.0176761
CGGGGAT45850.09.0117761
CGCGTTT9501.0633812E-88.99869251
TACAGAC114700.08.9845649
CCGGGAT46950.08.9017941
CGGAAAT34250.08.8746231
TCGTGTA129600.08.80913894-95
Produced by FastQC (version 0.11.5)