FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n02_WT_DI222_F2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n02_WT_DI222_F2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16212232
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA213950.015.5575562
GGGGGAG236650.011.4555083
ACATCTA105550.010.929714
GGGGAGA196500.010.6062684
AAAAGGG178750.010.1050878-79
CGGGAAT39900.09.9991091
AAGGGGG173800.09.96443880-81
CGCGGAT16800.09.8949521
ACCTTAC50600.09.6645936
CCCGTAT17250.09.3614851
CGCATAT10751.07320375E-109.2782431
AAAGGGG187850.09.08005180-81
ATCTACA123050.09.0674066
CCCACAT141750.08.97981
CGCCAAT24500.08.9175741
CGGCGAT21050.08.7996921
TAGGGGG57550.08.74311650-51
CGTCGTG182250.08.739223594-95
GCGTCGT199600.08.71688694-95
AACCTTA65500.08.6979995
Produced by FastQC (version 0.11.5)