FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n02_WT_DI222_F1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n02_WT_DI222_F1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17865726
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA260400.015.5530732
GGGGGAG270500.011.7940763
ACCTTAC56300.011.5531636
AACCTTA66150.011.1962175
CGCGTAT5606.29705E-711.0249181
AAAAGGG203400.010.94834778-79
GGGGAGA223200.010.8475864
AAGGGGG197350.010.41212380-81
CGCATAT12000.010.2899231
GCGGAAT23100.09.8684571
TAGGGGG69950.09.60179150-51
ACATCTA115350.09.548334
CGGGGAT59950.09.5063121
CCGGAAT48050.09.4885071
AAAGGGG212500.09.31207380-81
AAAAAAG366150.09.09051376-77
GGGGGTT110050.08.91611514-15
CGGGAAT43550.08.8331771
GGGGGGT233200.08.83295914-15
CGGGTAT18950.08.7716011
Produced by FastQC (version 0.11.5)