FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n02_KO_PR8_M2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n02_KO_PR8_M2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19345033
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA273900.013.45190052
ACATCTA121350.010.7191324
AAGGGGG211900.010.17955380-81
AAAAGGG222100.010.16619678-79
CGGCAAT29450.010.1601351
TAGGGGG74550.010.15536850-51
GGGGGAG295900.010.0597753
CGGGGAT62700.09.8473661
CCCACAT163450.09.6180561
CGCGAAT12550.09.4610761
CCGGGAT58700.09.3856451
ATCTACA143500.09.230566
CGGGAAT42100.09.0251031
CCGGAAT47700.08.9612461
CGCCGAT28200.08.757871
GGGGAGA233250.08.711064
ACCTTAC57250.08.6244946
CATCTAC155650.08.5401095
GGGGGGT250350.08.48380514-15
GCGTCGT187200.08.46984594-95
Produced by FastQC (version 0.11.5)