FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n02_KO_PR8_M1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n02_KO_PR8_M1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18839539
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA226500.014.2968692
GGGGGAG248550.09.9328263
TAGGGGG69800.09.75840650-51
GGGGAGA218850.09.6968964
CGGGTAT21950.09.0883941
CCGGAAT53550.09.0471771
ACATCTA118900.09.0239844
CGGGAAT43900.08.9801991
AAAGGGG205600.08.91959280-81
CCGGGAT56600.08.7274891
CGCGGAT17500.08.685291
AGGGGGG240200.08.56086382-83
GTCGATT18650.08.4044151
CCGGTAT22350.08.2881861
GGGGGGT195800.08.22646814-15
AAAAGGG198200.08.20193378-79
CGCGTAT7001.0410613E-48.142461
CGCGATT7603.059245E-58.1246021
AAGGGGG185650.08.06113980-81
TCGTGTA126750.08.05260494-95
Produced by FastQC (version 0.11.5)