FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n02_KO_PR8_F2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n02_KO_PR8_F2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18119005
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA220250.015.6507662
GGGGGAG237500.011.1140753
CGGGGAT58400.010.2467011
TAGGGGG63050.010.12557350-51
ACATCTA113700.09.8537264
GGGGAGA210400.09.8376124
CGGGAAT41500.09.498531
GCCGTAT18650.09.1674811
GGGGGGT169450.09.15537514-15
CCGGGAT53000.09.1400961
CGCGAAT11554.7293724E-119.046221
CCGGAAT46900.09.01246551
AAAGGGG199250.08.67999780-81
CCCACAT147850.08.5766231
AGGGGGG225150.08.43701282-83
AAGGGGG178950.08.43001980-81
CGCCAAT27900.08.3410031
GCGTCGT163950.08.26650894-95
AGCGTCG191450.08.25688594-95
CCGGTAT20750.08.2396891
Produced by FastQC (version 0.11.5)