FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n02_KO_PR8_F1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n02_KO_PR8_F1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18396546
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA228600.014.6645442
GGGGGAG249700.010.609663
TAGGGGG67000.010.59176550-51
ACATCTA121550.010.272444
GGGGGGT194550.09.62268514-15
GGGGAGA213550.09.4929484
AAGGGGG186750.09.3243480-81
ATCTACA135350.09.2255856
CCCACAT154100.09.1237241
CCGGTAT19450.09.0357691
AAAAGGG202250.09.01297878-79
CGCCAAT27450.08.9979591
CGGGAAT39100.08.9895521
CTCGTAT19600.08.9666191
AAAGGGG205300.08.852118580-81
CGCGAAA7653.5383619E-68.6925981
CGGGGAT55000.08.6361531
CGCCGAT25900.08.6194811
AGGGGGG244200.08.56665482-83
TACAGAC125950.08.5592619
Produced by FastQC (version 0.11.5)